Detailed information of TA system TA199193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 29323..29848
• Replicon: plasmid pKpCol17FII
• Accession: NZ_CP072907
• Organism: Klebsiella pneumoniae strain COL17

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: W8UQV6
• Locus tag: GQ627_RS28245
• Protein ID: WP_001568026.1
• Coordinates: 29543..29848 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: GQ627_RS28240
• Protein ID: WP_001568025.1
• Coordinates: 29323..29541 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ627_RS28195 (GQ627_0FII00025)	24713..24916	-	204	WP_004197809.1	hypothetical protein	-
GQ627_RS28200 (GQ627_0FII00026)	24930..25133	-	204	WP_004197808.1	HHA domain-containing protein	-
GQ627_RS28205 (GQ627_0FII00027)	25167..25535	-	369	WP_004197807.1	hypothetical protein	-
GQ627_RS28210 (GQ627_0FII00028)	25579..26073	-	495	WP_020805591.1	hypothetical protein	-
GQ627_RS28215 (GQ627_0FII00029)	26104..26676	-	573	WP_020805590.1	hypothetical protein	-
GQ627_RS28220 (GQ627_0FII00030)	26673..26921	-	249	WP_000272716.1	hypothetical protein	-
GQ627_RS28225 (GQ627_0FII00031)	27358..28047	+	690	WP_020315560.1	RES family NAD+ phosphorylase	-
GQ627_RS28230 (GQ627_0FII00032)	28079..28768	-	690	WP_004193995.1	hypothetical protein	-
GQ627_RS28235	28908..29153	+	246	Protein_31	hypothetical protein	-
GQ627_RS28240 (GQ627_0FII00033)	29323..29541	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
GQ627_RS28245 (GQ627_0FII00034)	29543..29848	+	306	WP_001568026.1	type II toxin-antitoxin system toxin CcdB	Toxin
GQ627_RS28250 (GQ627_0FII00035)	30017..30412	+	396	WP_072158608.1	hypothetical protein	-
GQ627_RS28255 (GQ627_0FII00036)	30439..30762	+	324	WP_022644730.1	hypothetical protein	-
GQ627_RS28260 (GQ627_0FII00037)	30759..31775	+	1017	WP_020315256.1	hypothetical protein	-
GQ627_RS28265 (GQ627_0FII00039)	32110..33378	-	1269	WP_019705993.1	ISL3-like element ISKpn25 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..100944	100944
	-	flank	IS/Tn	-	-	32110..33378	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11645.32 Da
• Isoelectric Point: 5.6919

>T199193 WP_001568026.1 NZ_CP072907:29543-29848 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T199193 NZ_CP097232:c1988330-1988013 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT199193 WP_001568025.1 NZ_CP072907:29323-29541 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT199193 NZ_CP097232:c1988020-1987733 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: A0A514EZN1

Antitoxin

• AlphaFold DB: A0A2J4ZZP8