Detailed information of TA system TA199191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 241607..242184
• Replicon: plasmid pKpCol17ndm
• Accession: NZ_CP072906
• Organism: Klebsiella pneumoniae strain COL17

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U5N651
• Locus tag: GQ627_RS27435
• Protein ID: WP_023159957.1
• Coordinates: 241607..241939 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GQ627_RS27440
• Protein ID: WP_096864992.1
• Coordinates: 241939..242184 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ627_RS27410 (GQ627_0ndm00281)	237755..238735	+	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
GQ627_RS27415 (GQ627_0ndm00282)	238884..239522	-	639	Protein_257	Tn3 family transposase	-
GQ627_RS27420 (GQ627_0ndm00283)	239805..240602	+	798	WP_048607643.1	hypothetical protein	-
GQ627_RS27425 (GQ627_0ndm00284)	240967..241110	+	144	WP_071547955.1	Hok/Gef family protein	-
GQ627_RS27430 (GQ627_0ndm00285)	241196..241576	-	381	WP_096864991.1	hypothetical protein	-
GQ627_RS27435 (GQ627_0ndm00286)	241607..241939	-	333	WP_023159957.1	endoribonuclease MazF	Toxin
GQ627_RS27440 (GQ627_0ndm00287)	241939..242184	-	246	WP_096864992.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GQ627_RS27445 (GQ627_0ndm00288)	242462..243085	+	624	WP_023159958.1	AAA family ATPase	-
GQ627_RS27450 (GQ627_0ndm00289)	243194..243421	+	228	WP_096864993.1	plasmid partition protein ParG	-
GQ627_RS27455 (GQ627_0ndm00290)	243519..243812	-	294	WP_101135438.1	hypothetical protein	-
GQ627_RS27460 (GQ627_0ndm00292)	244180..244455	+	276	WP_001141270.1	formaldehyde-responsive transcriptional repressor FrmR	-
GQ627_RS27465 (GQ627_0ndm00293)	244486..245595	+	1110	WP_101135439.1	S-(hydroxymethyl)glutathione dehydrogenase	-
GQ627_RS27470 (GQ627_0ndm00294)	245637..246035	+	399	WP_008813494.1	VOC family protein	-
GQ627_RS27475 (GQ627_0ndm00295)	246101..246937	+	837	WP_008813493.1	S-formylglutathione hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrE1 / aac(3)-IIa / blaTEM-1B / blaNDM-1 / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / catA1	-	1..372862	372862

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12097.88 Da
• Isoelectric Point: 7.0813

>T199191 WP_023159957.1 NZ_CP072906:c241939-241607 [Klebsiella pneumoniae]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Nucleotide

• Length: 333 bp

>T199191 NZ_CP097232:502330-503652 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCACCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACTCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8800.23 Da
• Isoelectric Point: 5.0508

>AT199191 WP_096864992.1 NZ_CP072906:c242184-241939 [Klebsiella pneumoniae]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLENLLAGITPENIHEKVDFGARTGKEL
I

Nucleotide

• Length: 246 bp

>AT199191 NZ_CP097232:502064-502330 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A2P1BQ75