Detailed information of TA system TA199190

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 195040..195793
• Replicon: plasmid pKpCol17ndm
• Accession: NZ_CP072906
• Organism: Klebsiella pneumoniae strain COL17

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: GQ627_RS27190
• Protein ID: WP_016154457.1
• Coordinates: 195416..195793 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: GQ627_RS27185
• Protein ID: WP_162838704.1
• Coordinates: 195040..195366 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ627_RS27125 (GQ627_0ndm00216)	190098..190364	+	267	WP_009652486.1	hypothetical protein	-
GQ627_RS27130 (GQ627_0ndm00217)	190377..190688	+	312	WP_009652410.1	hypothetical protein	-
GQ627_RS27135 (GQ627_0ndm00218)	190735..191205	+	471	WP_009652431.1	hypothetical protein	-
GQ627_RS27140 (GQ627_0ndm00219)	191254..191766	+	513	WP_009652395.1	DUF4234 domain-containing protein	-
GQ627_RS27145 (GQ627_0ndm00220)	191849..192070	+	222	WP_009652443.1	hypothetical protein	-
GQ627_RS27150 (GQ627_0ndm00221)	192126..192359	+	234	WP_009652474.1	DUF905 domain-containing protein	-
GQ627_RS27155 (GQ627_0ndm00222)	192463..193281	+	819	WP_009652424.1	DUF932 domain-containing protein	-
GQ627_RS27160 (GQ627_0ndm00223)	193281..193535	+	255	WP_009652519.1	hypothetical protein	-
GQ627_RS27165 (GQ627_0ndm00224)	193552..194025	+	474	WP_009652453.1	antirestriction protein	-
GQ627_RS27170 (GQ627_0ndm00225)	194036..194254	+	219	WP_009652503.1	hypothetical protein	-
GQ627_RS27175 (GQ627_0ndm00226)	194266..194748	+	483	WP_016154455.1	DNA repair protein RadC	-
GQ627_RS27180 (GQ627_0ndm00227)	194762..194983	+	222	WP_009652392.1	DUF987 domain-containing protein	-
GQ627_RS27185 (GQ627_0ndm00228)	195040..195366	+	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GQ627_RS27190 (GQ627_0ndm00229)	195416..195793	+	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
GQ627_RS27195 (GQ627_0ndm00230)	195790..196281	+	492	WP_009652438.1	DUF5983 family protein	-
GQ627_RS27200 (GQ627_0ndm00231)	196311..196514	+	204	WP_009652505.1	DUF957 domain-containing protein	-
GQ627_RS27205 (GQ627_0ndm00233)	196595..197440	+	846	Protein_215	DUF4942 domain-containing protein	-
GQ627_RS27210 (GQ627_0ndm00235)	197640..198671	+	1032	WP_223175620.1	hypothetical protein	-
GQ627_RS27215 (GQ627_0ndm00236)	198674..199147	+	474	WP_009652494.1	ribbon-helix-helix protein, CopG family	-
GQ627_RS27220 (GQ627_0ndm00237)	199467..200105	+	639	WP_009652437.1	VWA domain-containing protein	-
GQ627_RS27225 (GQ627_0ndm00238)	200130..200771	+	642	WP_009652489.1	TerD family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrE1 / aac(3)-IIa / blaTEM-1B / blaNDM-1 / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / catA1	-	1..372862	372862

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T199190 WP_016154457.1 NZ_CP072906:195416-195793 [Klebsiella pneumoniae]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T199190 NZ_CP097230:30343-30759 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT199190 WP_162838704.1 NZ_CP072906:195040-195366 [Klebsiella pneumoniae]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT199190 NZ_CP097230:30116-30346 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: R8WN26