Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 37400..38310 Replicon plasmid pKpCol17ndm
Accession NZ_CP072906
Organism Klebsiella pneumoniae strain COL17

Toxin (Protein)


Gene name - Uniprot ID A0A1D8K2L2
Locus tag GQ627_RS26380 Protein ID WP_070083042.1
Coordinates 37840..38310 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag GQ627_RS26375 Protein ID WP_004181895.1
Coordinates 37400..37843 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ627_RS26360 (GQ627_0ndm00054) 34033..35520 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
GQ627_RS26365 (GQ627_0ndm00055) 35606..36028 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
GQ627_RS26370 (GQ627_0ndm00056) 36028..37299 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
GQ627_RS26375 (GQ627_0ndm00057) 37400..37843 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
GQ627_RS26380 (GQ627_0ndm00058) 37840..38310 + 471 WP_070083042.1 RES family NAD+ phosphorylase Toxin
GQ627_RS26385 (GQ627_0ndm00059) 38421..38681 - 261 WP_070083041.1 hypothetical protein -
GQ627_RS26390 39371..40740 + 1370 WP_085956500.1 IS3-like element ISKpn8 family transposase -
GQ627_RS26395 (GQ627_0ndm00062) 40919..42259 + 1341 WP_000589001.1 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrE1 / aac(3)-IIa / blaTEM-1B / blaNDM-1 / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / catA1 - 1..372862 372862
- inside IScluster/Tn - - 39371..51570 12199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17587.85 Da        Isoelectric Point: 4.5244

>T199188 WP_070083042.1 NZ_CP072906:37840-38310 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMESFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T199188 NZ_CP097229:137925-138209 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT199188 WP_004181895.1 NZ_CP072906:37400-37843 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT199188 NZ_CP097229:137687-137935 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8K2L2

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References