Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4900081..4900817 Replicon chromosome
Accession NZ_CP072905
Organism Klebsiella pneumoniae strain COL17

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag GQ627_RS23990 Protein ID WP_032433360.1
Coordinates 4900335..4900817 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag GQ627_RS23985 Protein ID WP_003026799.1
Coordinates 4900081..4900347 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ627_RS23960 (4895727) 4895727..4896866 + 1140 WP_032433368.1 mannitol dehydrogenase -
GQ627_RS23965 (4896895) 4896895..4897557 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
GQ627_RS23970 (4897541) 4897541..4898545 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
GQ627_RS23975 (4898563) 4898563..4899195 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
GQ627_RS23980 (4899205) 4899205..4899768 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
GQ627_RS23985 (4900081) 4900081..4900347 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
GQ627_RS23990 (4900335) 4900335..4900817 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
GQ627_RS23995 (4901178) 4901178..4901777 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
GQ627_RS24000 (4901990) 4901990..4902934 - 945 WP_077254249.1 fimbrial protein -
GQ627_RS24005 (4902946) 4902946..4903524 - 579 WP_032432061.1 type 1 fimbrial protein -
GQ627_RS24010 (4903528) 4903528..4904268 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T199187 WP_032433360.1 NZ_CP072905:4900335-4900817 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T199187 NZ_CP097229:19857-20339 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT199187 WP_003026799.1 NZ_CP072905:4900081-4900347 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT199187 NZ_CP097229:19588-19866 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References