Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3341444..3342069 Replicon chromosome
Accession NZ_CP072905
Organism Klebsiella pneumoniae strain COL17

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GQ627_RS16305 Protein ID WP_002882817.1
Coordinates 3341444..3341827 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GQ627_RS16310 Protein ID WP_004150355.1
Coordinates 3341827..3342069 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQ627_RS16290 (3338810) 3338810..3339712 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GQ627_RS16295 (3339709) 3339709..3340344 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GQ627_RS16300 (3340341) 3340341..3341270 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GQ627_RS16305 (3341444) 3341444..3341827 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GQ627_RS16310 (3341827) 3341827..3342069 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
GQ627_RS16315 (3342274) 3342274..3343191 + 918 WP_021466676.1 alpha/beta hydrolase -
GQ627_RS16320 (3343205) 3343205..3344146 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GQ627_RS16325 (3344191) 3344191..3344628 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
GQ627_RS16330 (3344625) 3344625..3345485 - 861 WP_004146232.1 virulence factor BrkB family protein -
GQ627_RS16335 (3345479) 3345479..3346078 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T199182 WP_002882817.1 NZ_CP072905:c3341827-3341444 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T199182 NZ_CP097228:c3031202-3030918 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT199182 WP_004150355.1 NZ_CP072905:c3342069-3341827 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT199182 NZ_CP097228:c3031434-3031192 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References