Detailed information of TA system TA199132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 262420..263009
• Replicon: chromosome
• Accession: NZ_CP072901
• Organism: Bartonella henselae strain Berlin-I

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: X5M710
• Locus tag: KAE72_RS01230
• Protein ID: WP_011180620.1
• Coordinates: 262420..262752 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A829Z4P4
• Locus tag: KAE72_RS01235
• Protein ID: WP_034448603.1
• Coordinates: 262752..263009 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAE72_RS01200 (KAE72_01185)	258925..260250	-	1326	WP_011180823.1	phage terminase large subunit	-
KAE72_RS01205 (KAE72_01190)	260231..260557	-	327	WP_011180626.1	hypothetical protein	-
KAE72_RS01210 (KAE72_01195)	260856..261314	+	459	WP_011180624.1	hypothetical protein	-
KAE72_RS01215	261410..261802	+	393	WP_075320637.1	hypothetical protein	-
KAE72_RS01220 (KAE72_01200)	261832..262101	-	270	WP_011180622.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KAE72_RS01225 (KAE72_01205)	262085..262312	-	228	WP_007346764.1	DUF6290 family protein	-
KAE72_RS01230 (KAE72_01210)	262420..262752	-	333	WP_011180620.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KAE72_RS01235 (KAE72_01215)	262752..263009	-	258	WP_034448603.1	antitoxin	Antitoxin
KAE72_RS01240 (KAE72_01220)	263090..263584	-	495	WP_059443338.1	hypothetical protein	-
KAE72_RS01245 (KAE72_01225)	263638..264084	-	447	WP_011180617.1	single-stranded DNA-binding protein	-
KAE72_RS01250 (KAE72_01230)	264296..264586	+	291	WP_011180616.1	hypothetical protein	-
KAE72_RS01255 (KAE72_01235)	264583..264855	+	273	WP_011180615.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
KAE72_RS01260 (KAE72_01240)	265046..265363	-	318	Protein_242	hypothetical protein	-
KAE72_RS01265 (KAE72_01245)	265545..265931	-	387	WP_082246570.1	hypothetical protein	-
KAE72_RS01270 (KAE72_01250)	265915..267165	-	1251	WP_011180612.1	DUF1376 domain-containing protein	-
KAE72_RS01275 (KAE72_01255)	267155..267634	-	480	WP_011180611.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	235927..276049	40122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11865.88 Da
• Isoelectric Point: 10.5590

>T199132 WP_011180620.1 NZ_CP072901:c262752-262420 [Bartonella henselae]
MKRGEIWLVSLDPTSGYEQKGTRPVLIVSPEAFNRVTKTPIVLPITSGGNFSRTAGFAVSLVGTGLHTTGVIRCDQPRAL
DIAARQGKKMETVPPIIMNEVLAKLSTFLT

Nucleotide

• Length: 333 bp

>T199132 NZ_CP097213:1900035-1900289 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCTCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCTACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9381.71 Da
• Isoelectric Point: 4.2101

>AT199132 WP_034448603.1 NZ_CP072901:c263009-262752 [Bartonella henselae]
MHTTKLRKVGGSVMLSIPPALLDVLHLTENTQVGLAVDNGQLVVKPQTFPSYTLDELLAQCNPSDDFADEDMEWFDAKPV
GRELL

Nucleotide

• Length: 258 bp

>AT199132 NZ_CP097213:1899787-1900038 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A067W4K9

Antitoxin

• AlphaFold DB: A0A829Z4P4