Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-pasA/PRK09812-HTH
Location 123173..123840 Replicon chromosome
Accession NZ_CP072897
Organism Bartonella henselae strain FR96/BK3

Toxin (Protein)


Gene name pemK Uniprot ID X5M710
Locus tag KAE70_RS00520 Protein ID WP_011180620.1
Coordinates 123173..123505 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pasA Uniprot ID N6VJG4
Locus tag KAE70_RS00525 Protein ID WP_007346764.1
Coordinates 123613..123840 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KAE70_RS00465 (KAE70_00460) 118271..118792 + 522 WP_236095459.1 hypothetical protein -
KAE70_RS00470 (KAE70_00465) 118976..119311 + 336 WP_082250410.1 hypothetical protein -
KAE70_RS00475 119355..119870 + 516 Protein_93 hypothetical protein -
KAE70_RS00480 (KAE70_00470) 119988..120416 - 429 WP_082250673.1 type II toxin-antitoxin system HicB family antitoxin -
KAE70_RS00485 (KAE70_00475) 120490..120795 - 306 WP_082250408.1 BrnA antitoxin family protein -
KAE70_RS00490 (KAE70_00480) 120773..121072 - 300 WP_034448610.1 BrnT family toxin -
KAE70_RS00495 (KAE70_00485) 121166..121489 - 324 WP_082250693.1 type II toxin-antitoxin system PemK/MazF family toxin -
KAE70_RS00500 (KAE70_00490) 121489..121713 - 225 WP_038487033.1 antitoxin MazE family protein -
KAE70_RS00505 (KAE70_00495) 121836..122267 + 432 WP_082250672.1 single-stranded DNA-binding protein -
KAE70_RS00510 (KAE70_00500) 122323..122835 + 513 WP_172642310.1 hypothetical protein -
KAE70_RS00515 (KAE70_00505) 122916..123173 + 258 WP_034448603.1 antitoxin -
KAE70_RS00520 (KAE70_00510) 123173..123505 + 333 WP_011180620.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KAE70_RS00525 (KAE70_00515) 123613..123840 + 228 WP_007346764.1 DUF6290 family protein Antitoxin
KAE70_RS00530 (KAE70_00520) 123824..124093 + 270 WP_011180622.1 type II toxin-antitoxin system RelE/ParE family toxin -
KAE70_RS00535 124123..124515 - 393 WP_223396448.1 hypothetical protein -
KAE70_RS00540 (KAE70_00525) 124850..125176 + 327 WP_011180626.1 hypothetical protein -
KAE70_RS00545 (KAE70_00530) 125157..126482 + 1326 WP_082250858.1 phage terminase large subunit -
KAE70_RS00550 (KAE70_00535) 126467..128521 + 2055 WP_011180822.1 portal protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 111262..176233 64971


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin

Protein of unknown function DUF6290

(2-71)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11865.88 Da        Isoelectric Point: 10.5590

>T199091 WP_011180620.1 NZ_CP072897:123173-123505 [Bartonella henselae]
MKRGEIWLVSLDPTSGYEQKGTRPVLIVSPEAFNRVTKTPIVLPITSGGNFSRTAGFAVSLVGTGLHTTGVIRCDQPRAL
DIAARQGKKMETVPPIIMNEVLAKLSTFLT

Download         Length: 333 bp

>T199091 NZ_CP097181:2384320-2385642 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATTACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGTGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8742.78 Da        Isoelectric Point: 4.8780

>AT199091 WP_007346764.1 NZ_CP072897:123613-123840 [Bartonella henselae]
MTISIRLPSDLETRLNNLAAKTGRTKSFYLREIIERGIEEAEDYYLASQVRERVRRGEGTFYSSEEVRKELGLDD

Download         Length: 228 bp

>AT199091 NZ_CP097181:2384054-2384320 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A067W4K9

Antitoxin

Source ID Structure
AlphaFold DB N6VJG4

References