Detailed information of TA system TA199090

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 122916..123505
• Replicon: chromosome
• Accession: NZ_CP072897
• Organism: Bartonella henselae strain FR96/BK3

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: X5M710
• Locus tag: KAE70_RS00520
• Protein ID: WP_011180620.1
• Coordinates: 123173..123505 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A829Z4P4
• Locus tag: KAE70_RS00515
• Protein ID: WP_034448603.1
• Coordinates: 122916..123173 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAE70_RS08380	118013..118144	+	132	WP_268885550.1	hypothetical protein	-
KAE70_RS00465 (KAE70_00460)	118271..118792	+	522	WP_236095459.1	hypothetical protein	-
KAE70_RS00470 (KAE70_00465)	118976..119311	+	336	WP_082250410.1	hypothetical protein	-
KAE70_RS00475	119355..119870	+	516	Protein_93	hypothetical protein	-
KAE70_RS00480 (KAE70_00470)	119988..120416	-	429	WP_082250673.1	type II toxin-antitoxin system HicB family antitoxin	-
KAE70_RS00485 (KAE70_00475)	120490..120795	-	306	WP_082250408.1	BrnA antitoxin family protein	-
KAE70_RS00490 (KAE70_00480)	120773..121072	-	300	WP_034448610.1	BrnT family toxin	-
KAE70_RS00495 (KAE70_00485)	121166..121489	-	324	WP_082250693.1	type II toxin-antitoxin system PemK/MazF family toxin	-
KAE70_RS00500 (KAE70_00490)	121489..121713	-	225	WP_038487033.1	antitoxin MazE family protein	-
KAE70_RS00505 (KAE70_00495)	121836..122267	+	432	WP_082250672.1	single-stranded DNA-binding protein	-
KAE70_RS00510 (KAE70_00500)	122323..122835	+	513	WP_172642310.1	hypothetical protein	-
KAE70_RS00515 (KAE70_00505)	122916..123173	+	258	WP_034448603.1	antitoxin	Antitoxin
KAE70_RS00520 (KAE70_00510)	123173..123505	+	333	WP_011180620.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KAE70_RS00525 (KAE70_00515)	123613..123840	+	228	WP_007346764.1	DUF6290 family protein	-
KAE70_RS00530 (KAE70_00520)	123824..124093	+	270	WP_011180622.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KAE70_RS00535	124123..124515	-	393	WP_223396448.1	hypothetical protein	-
KAE70_RS00540 (KAE70_00525)	124850..125176	+	327	WP_011180626.1	hypothetical protein	-
KAE70_RS00545 (KAE70_00530)	125157..126482	+	1326	WP_082250858.1	phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	111262..176233	64971

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11865.88 Da
• Isoelectric Point: 10.5590

>T199090 WP_011180620.1 NZ_CP072897:123173-123505 [Bartonella henselae]
MKRGEIWLVSLDPTSGYEQKGTRPVLIVSPEAFNRVTKTPIVLPITSGGNFSRTAGFAVSLVGTGLHTTGVIRCDQPRAL
DIAARQGKKMETVPPIIMNEVLAKLSTFLT

Nucleotide

• Length: 333 bp

>T199090 NZ_CP097181:2057976-2058079 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9381.71 Da
• Isoelectric Point: 4.2101

>AT199090 WP_034448603.1 NZ_CP072897:122916-123173 [Bartonella henselae]
MHTTKLRKVGGSVMLSIPPALLDVLHLTENTQVGLAVDNGQLVVKPQTFPSYTLDELLAQCNPSDDFADEDMEWFDAKPV
GRELL

Nucleotide

• Length: 258 bp

>AT199090 NZ_CP097181:c2058117-2057846 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A067W4K9

Antitoxin

• AlphaFold DB: A0A829Z4P4