Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 23347..24484 Replicon plasmid pAVS02435_2
Accession NZ_CP072896
Organism Enterococcus faecium strain AVS0243

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag J9543_RS12890 Protein ID WP_001284311.1
Coordinates 23347..24210 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag J9543_RS12895 Protein ID WP_000301765.1
Coordinates 24212..24484 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9543_RS12870 (J9543_12865) 18435..18845 + 411 WP_218210702.1 hypothetical protein -
J9543_RS12875 (J9543_12870) 19004..20917 + 1914 WP_231416096.1 ABC-F type ribosomal protection protein OptrA -
J9543_RS12880 (J9543_12875) 21137..21919 + 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
J9543_RS12885 (J9543_12880) 22083..23084 + 1002 WP_231415008.1 23S ribosomal RNA methyltransferase Erm -
J9543_RS12890 (J9543_12885) 23347..24210 - 864 WP_001284311.1 zeta toxin family protein Toxin
J9543_RS12895 (J9543_12890) 24212..24484 - 273 WP_000301765.1 antitoxin Antitoxin
J9543_RS12900 (J9543_12895) 24501..24716 - 216 WP_001835296.1 peptide-binding protein -
J9543_RS12905 (J9543_12900) 24808..25704 - 897 WP_001835294.1 ParA family protein -
J9543_RS12910 (J9543_12905) 25807..27951 - 2145 WP_000108741.1 type IA DNA topoisomerase -
J9543_RS12915 (J9543_12910) 27951..28568 - 618 WP_001062589.1 recombinase family protein -
J9543_RS12920 (J9543_12915) 28582..28752 - 171 WP_000713594.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) / fexA / optrA / ant(9)-Ia - 1..36447 36447


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T199088 WP_001284311.1 NZ_CP072896:c24210-23347 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T199088 NZ_CP097181:1019743-1020078 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT199088 WP_000301765.1 NZ_CP072896:c24484-24212 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT199088 NZ_CP097181:1019495-1019743 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References