Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2127035..2127606 Replicon chromosome
Accession NZ_CP072894
Organism Enterococcus faecium strain AVS0243

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag J9543_RS10225 Protein ID WP_002302307.1
Coordinates 2127035..2127376 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag J9543_RS10230 Protein ID WP_002323011.1
Coordinates 2127376..2127606 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9543_RS10215 (J9543_10210) 2122065..2124569 - 2505 WP_002317782.1 prealbumin-like fold domain-containing protein -
J9543_RS10220 (J9543_10215) 2124900..2126062 + 1163 WP_086956687.1 IS3 family transposase -
J9543_RS10225 (J9543_10220) 2127035..2127376 - 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J9543_RS10230 (J9543_10225) 2127376..2127606 - 231 WP_002323011.1 hypothetical protein Antitoxin
J9543_RS10235 (J9543_10230) 2127894..2128232 + 339 WP_002286804.1 hypothetical protein -
J9543_RS10240 (J9543_10235) 2128437..2129012 - 576 WP_002302305.1 SOS response-associated peptidase family protein -
J9543_RS10245 (J9543_10240) 2129376..2129957 - 582 WP_002300332.1 TetR/AcrR family transcriptional regulator -
J9543_RS10250 (J9543_10245) 2130150..2130776 - 627 WP_002300333.1 cysteine hydrolase -
J9543_RS10255 (J9543_10250) 2130798..2132129 - 1332 WP_002300334.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T199087 WP_002302307.1 NZ_CP072894:c2127376-2127035 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T199087 NZ_CP097181:893540-893947 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTGATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT199087 WP_002323011.1 NZ_CP072894:c2127606-2127376 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT199087 NZ_CP097181:893293-893559 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCAGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References