Detailed information of TA system TA199083

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 23554..24662
• Replicon: plasmid pF162_2_2
• Accession: NZ_CP072890
• Organism: Enterococcus raffinosus strain F162_2

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: J9537_RS20455
• Protein ID: WP_000233000.1
• Coordinates: 23793..24662 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: J9537_RS20450
• Protein ID: WP_000205227.1
• Coordinates: 23554..23778 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9537_RS20415 (J9537_20415)	18570..18779	+	210	WP_218257691.1	hypothetical protein	-
J9537_RS20420 (J9537_20420)	19143..19313	+	171	WP_000713595.1	hypothetical protein	-
J9537_RS20425 (J9537_20425)	19327..19929	+	603	WP_218257685.1	recombinase family protein	-
J9537_RS20430 (J9537_20430)	19945..20622	+	678	Protein_24	DNA topoisomerase	-
J9537_RS20755	20933..21430	+	498	WP_002327635.1	DNA recombinase	-
J9537_RS20440 (J9537_20440)	21431..21847	+	417	WP_000323438.1	recombinase	-
J9537_RS20445 (J9537_20445)	21849..23411	+	1563	WP_000136908.1	recombinase family protein	-
J9537_RS20450 (J9537_20450)	23554..23778	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
J9537_RS20455 (J9537_20455)	23793..24662	+	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
J9537_RS20460 (J9537_20460)	24643..25377	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
J9537_RS20465 (J9537_20465)	25410..26273	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
J9537_RS20470 (J9537_20470)	26317..26844	+	528	WP_002294507.1	phosphoribosyltransferase family protein	-
J9537_RS20475 (J9537_20475)	26976..27785	+	810	WP_002294509.1	ANT(9) family aminoglycoside nucleotidyltransferase Spw	-
J9537_RS20480 (J9537_20480)	27964..28254	+	291	WP_010733534.1	hypothetical protein	-
J9537_RS20485 (J9537_20485)	28305..28763	-	459	WP_002325145.1	hypothetical protein	-
J9537_RS20490 (J9537_20490)	28846..29394	+	549	Protein_36	recombinase zinc beta ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	dfrG / ant(6)-Ia / lsa(E) / lnu(B) / aph(3')-III / erm(B)	-	1..37686	37686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T199083 WP_000233000.1 NZ_CP072890:23793-24662 [Enterococcus raffinosus]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T199083 NZ_CP097181:205480-205587 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT199083 WP_000205227.1 NZ_CP072890:23554-23778 [Enterococcus raffinosus]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT199083 NZ_CP097181:c205432-205366 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: R3JIN1