Detailed information of TA system TA199081

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/-
• Location: 689294..690372
• Replicon: plasmid pF162_2_1
• Accession: NZ_CP072889
• Organism: Enterococcus raffinosus strain F162_2

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: -
• Locus tag: J9537_RS17890
• Protein ID: WP_218257386.1
• Coordinates: 689294..689506 (-)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: -
• Locus tag: J9537_RS17895
• Protein ID: WP_218257387.1
• Coordinates: 689503..690372 (-)
• Length: 290 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9537_RS17870 (J9537_17870)	684840..685454	-	615	WP_218257383.1	CPBP family intramembrane metalloprotease	-
J9537_RS17875 (J9537_17875)	685468..685779	-	312	WP_218257384.1	heavy metal-binding domain-containing protein	-
J9537_RS17880 (J9537_17880)	686502..688157	+	1656	WP_245001324.1	IS1182 family transposase	-
J9537_RS17885 (J9537_17885)	688623..689309	-	687	WP_218257385.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
J9537_RS17890 (J9537_17890)	689294..689506	-	213	WP_218257386.1	hypothetical protein	Toxin
J9537_RS17895 (J9537_17895)	689503..690372	-	870	WP_218257387.1	hypothetical protein	Antitoxin
J9537_RS20820	690676..690798	+	123	WP_280640442.1	hypothetical protein	-
J9537_RS17900 (J9537_17900)	691040..691321	-	282	WP_218257388.1	hypothetical protein	-
J9537_RS17905 (J9537_17905)	691689..691970	-	282	WP_245001325.1	DUF4176 domain-containing protein	-
J9537_RS17910 (J9537_17910)	691975..692874	-	900	WP_218257389.1	hypothetical protein	-
J9537_RS17915 (J9537_17915)	693259..693810	-	552	WP_218257390.1	hypothetical protein	-
J9537_RS17920 (J9537_17920)	693869..694777	-	909	WP_218257391.1	hypothetical protein	-
J9537_RS17925 (J9537_17925)	694878..695258	-	381	WP_245001362.1	suppressor of fused domain protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1186145	1186145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8203.78 Da
• Isoelectric Point: 10.6919

>T199081 WP_218257386.1 NZ_CP072889:c689506-689294 [Enterococcus raffinosus]
MNSDRLRDLIKAKKGSIASAEELRIRYATERFLLRVQASPYKDKLILKGGFLLGTIYKIEIERPKIWMHL

Nucleotide

• Length: 213 bp

>T199081 NZ_CP097172:2255-2533 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
TGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 290 a.a.
• Molecular weight: 33291.79 Da
• Isoelectric Point: 7.1307

>AT199081 WP_218257387.1 NZ_CP072889:c690372-689503 [Enterococcus raffinosus]
MTNRSNLNHSEQINRLLDTVKEMNPEALKDVQEFLEQLTLGDRLSRYIDFDELPAEDTDEFTFLKLVADSKGIIVKKRLD
KVGLSHYKSKQFLSKYGFQSMYGGSLYISPKNSLDGVYIFQQQYSKAVISHETALYFHDLTDVIPKTTIMSLPKTYNLNQ
LFVWDGESPHLTVSKAPSTFHNKVRVIVHYSGNDSIDIVRNNEIKSSEIETFETTFGNQVRVTTPERTIADILKTSSYTE
AEVMFDAVKRYMNNPQSKELRLRRIADEQGVKKRLDECLTLLRSRGEIK

Nucleotide

• Length: 870 bp

>AT199081 NZ_CP097172:1986-2255 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA