Detailed information of TA system TA199080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 832294..832919
• Replicon: chromosome
• Accession: NZ_CP072888
• Organism: Enterococcus raffinosus strain F162_2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: R2RF59
• Locus tag: J9537_RS04125
• Protein ID: WP_010746644.1
• Coordinates: 832734..832919 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J9537_RS04120
• Protein ID: WP_218256818.1
• Coordinates: 832294..832680 (-)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9537_RS04095 (J9537_04095)	827919..828254	+	336	WP_218256814.1	hypothetical protein	-
J9537_RS04100 (J9537_04100)	828624..829097	+	474	WP_218256815.1	hypothetical protein	-
J9537_RS04105 (J9537_04105)	829101..830315	+	1215	WP_218256816.1	biotin/lipoyl-binding protein	-
J9537_RS04110 (J9537_04110)	830316..831002	+	687	WP_010746647.1	ABC transporter ATP-binding protein	-
J9537_RS04115 (J9537_04115)	830999..832198	+	1200	WP_218256817.1	ABC transporter permease	-
J9537_RS04120 (J9537_04120)	832294..832680	-	387	WP_218256818.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J9537_RS04125 (J9537_04125)	832734..832919	-	186	WP_010746644.1	type II toxin-antitoxin system HicA family toxin	Toxin
J9537_RS04130 (J9537_04130)	833153..834088	-	936	WP_218256819.1	membrane protein insertase YidC	-
J9537_RS04135 (J9537_04135)	834193..834465	-	273	WP_010746642.1	acylphosphatase	-
J9537_RS04140 (J9537_04140)	834605..835366	+	762	WP_218256820.1	RNA methyltransferase	-
J9537_RS04145 (J9537_04145)	835497..835994	+	498	WP_010746640.1	HD domain-containing protein	-
J9537_RS04150 (J9537_04150)	836365..836997	+	633	WP_218256821.1	YitT family protein	-
J9537_RS04155 (J9537_04155)	837170..837733	+	564	WP_010746638.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (12-93)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 7029.19 Da
• Isoelectric Point: 10.5745

>T199080 WP_010746644.1 NZ_CP072888:c832919-832734 [Enterococcus raffinosus]
MDAKELAKILKNDGWYFDSQRGSHRYYKHPIKKGKVPVPFHSHKDIPIGTLNNILKQAGLK

Nucleotide

• Length: 186 bp

>T199080 NZ_CP072888:c832919-832734 [Enterococcus raffinosus]
ATGGATGCTAAGGAGCTAGCCAAGATTCTTAAAAACGACGGTTGGTATTTTGATTCTCAACGTGGAAGTCATCGCTACTA
CAAACATCCCATAAAAAAGGGCAAAGTACCTGTCCCATTCCACAGTCACAAAGACATCCCTATTGGAACACTCAACAACA
TACTTAAACAAGCTGGCTTAAAGTAG

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14256.25 Da
• Isoelectric Point: 4.3857

>AT199080 WP_218256818.1 NZ_CP072888:c832680-832294 [Enterococcus raffinosus]
VVIYYAVFDFADDGINVVFPDLNNAATFGENMHEALYMAKDLLAGWLIDAEDEKQAFPSPTDHRSLSVAAGNLLIPIEVD
LSFYRKKFESKPIKKTLTIPKYLNDLGNEAGINFSATLTEALKEKLEV

Nucleotide

• Length: 387 bp

>AT199080 NZ_CP072888:c832680-832294 [Enterococcus raffinosus]
GTGGTCATTTATTATGCTGTGTTCGATTTTGCAGATGATGGGATCAATGTTGTTTTTCCAGATTTGAACAATGCGGCTAC
ATTTGGTGAAAATATGCATGAGGCCCTCTATATGGCTAAAGATTTACTAGCAGGTTGGCTGATTGATGCTGAGGATGAAA
AGCAAGCTTTTCCTTCACCTACTGATCACCGAAGTCTGAGTGTAGCAGCTGGAAATTTATTGATCCCAATTGAAGTTGAT
TTATCCTTTTACCGTAAAAAATTTGAAAGTAAGCCTATCAAAAAAACATTAACTATTCCAAAATATTTAAATGATTTAGG
CAATGAAGCAGGAATCAACTTCTCAGCTACACTAACTGAAGCATTGAAGGAAAAATTAGAAGTTTAG

Structures

Toxin

• AlphaFold DB: R2RF59