Detailed information of TA system TA199079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 11460..12597
• Replicon: plasmid pF179_2
• Accession: NZ_CP072886
• Organism: Enterococcus faecium strain F179

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: J9540_RS13425
• Protein ID: WP_024417198.1
• Coordinates: 11460..12323 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: J9540_RS13430
• Protein ID: WP_000301765.1
• Coordinates: 12325..12597 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9540_RS13400 (J9540_13400)	6684..7259	+	576	WP_230307287.1	IS30 family transposase	-
J9540_RS13405 (J9540_13405)	7331..8944	-	1614	WP_098379807.1	tetronasin resistance protein	-
J9540_RS13410 (J9540_13410)	8968..9849	-	882	WP_098379806.1	ABC transporter ATP-binding protein	-
J9540_RS13415 (J9540_13415)	10160..10756	+	597	WP_002326773.1	TetR/AcrR family transcriptional regulator	-
J9540_RS13420 (J9540_13420)	10925..11329	-	405	Protein_13	molecular chaperone DnaJ	-
J9540_RS13425 (J9540_13425)	11460..12323	-	864	WP_024417198.1	zeta toxin family protein	Toxin
J9540_RS13430 (J9540_13430)	12325..12597	-	273	WP_000301765.1	antitoxin	Antitoxin
J9540_RS13435 (J9540_13435)	12614..12829	-	216	WP_001835296.1	peptide-binding protein	-
J9540_RS13440 (J9540_13440)	12893..13789	-	897	WP_001835294.1	ParA family protein	-
J9540_RS13445 (J9540_13445)	13892..16036	-	2145	WP_000108741.1	type IA DNA topoisomerase	-
J9540_RS13450 (J9540_13450)	16036..16653	-	618	WP_001062589.1	recombinase family protein	-
J9540_RS13455 (J9540_13455)	16667..16837	-	171	WP_000713594.1	hypothetical protein	-
J9540_RS13460 (J9540_13460)	17282..17596	+	315	WP_000420682.1	YdcP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	lnu(B) / aph(3')-III / erm(B) / tet(M) / tet(L) / ant(6)-Ia	-	1..53837	53837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32404.02 Da
• Isoelectric Point: 6.9964

>T199079 WP_024417198.1 NZ_CP072886:c12323-11460 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T199079 NZ_CP097170:c4740708-4740334 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT199079 WP_000301765.1 NZ_CP072886:c12597-12325 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT199079 NZ_CP097170:c4740950-4740708 [Escherichia coli]
ATGAGCGCTATGGCAGGTATGGATATGGGCAGAATTTTACTCGATTTATCCGATGAGGTGATTAAGCGACTTGATGATCT
CAAAGTGCAGCGCAATCTTCCTCGCGCAGAGCTATTACGAGAAGCTGTAGATCAATATCTGGAAAATCAATCGAAAACGA
CAATATCCAGTGCTTTTGGTATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3