Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 11460..12597 Replicon plasmid pF179_2
Accession NZ_CP072886
Organism Enterococcus faecium strain F179

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag J9540_RS13425 Protein ID WP_024417198.1
Coordinates 11460..12323 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag J9540_RS13430 Protein ID WP_000301765.1
Coordinates 12325..12597 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9540_RS13400 (J9540_13400) 6684..7259 + 576 WP_230307287.1 IS30 family transposase -
J9540_RS13405 (J9540_13405) 7331..8944 - 1614 WP_098379807.1 tetronasin resistance protein -
J9540_RS13410 (J9540_13410) 8968..9849 - 882 WP_098379806.1 ABC transporter ATP-binding protein -
J9540_RS13415 (J9540_13415) 10160..10756 + 597 WP_002326773.1 TetR/AcrR family transcriptional regulator -
J9540_RS13420 (J9540_13420) 10925..11329 - 405 Protein_13 molecular chaperone DnaJ -
J9540_RS13425 (J9540_13425) 11460..12323 - 864 WP_024417198.1 zeta toxin family protein Toxin
J9540_RS13430 (J9540_13430) 12325..12597 - 273 WP_000301765.1 antitoxin Antitoxin
J9540_RS13435 (J9540_13435) 12614..12829 - 216 WP_001835296.1 peptide-binding protein -
J9540_RS13440 (J9540_13440) 12893..13789 - 897 WP_001835294.1 ParA family protein -
J9540_RS13445 (J9540_13445) 13892..16036 - 2145 WP_000108741.1 type IA DNA topoisomerase -
J9540_RS13450 (J9540_13450) 16036..16653 - 618 WP_001062589.1 recombinase family protein -
J9540_RS13455 (J9540_13455) 16667..16837 - 171 WP_000713594.1 hypothetical protein -
J9540_RS13460 (J9540_13460) 17282..17596 + 315 WP_000420682.1 YdcP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid lnu(B) / aph(3')-III / erm(B) / tet(M) / tet(L) / ant(6)-Ia - 1..53837 53837


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T199079 WP_024417198.1 NZ_CP072886:c12323-11460 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

>T199079 NZ_CP097170:c4740708-4740334 [Escherichia coli]
ATGGCAAAAAGAACTGCGCTTTTTGATACCAATATCCTTATCGATTTGTTCAGCGGACGAATTGAGGCAAAACAGGCTCT
GGAGGCATGGCCCCTACAGAATGCGATTAGTCTGGTCACATGGATGGAGGTGCTGGTTGGCGCAAAAAAATATGATCAAG
AACATCGCACACGGATTGCAATGAGTGCGTTTAACGTTATTGGGATTTCGCAGGATATCGCGGAACGAAGCGTTGCTTTA
AGGCAGGAGTATAGAATGAAACTTCCAGATGCCATTATTCTGGCAACAGCACAGATTTATGGCTTTGAACTGGTAACGCG
AAATACGAGAGATTTTGCGGGAATAGCAGGTGTCGTCACACCCTATGAGCTGTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT199079 WP_000301765.1 NZ_CP072886:c12597-12325 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT199079 NZ_CP097170:c4740950-4740708 [Escherichia coli]
ATGAGCGCTATGGCAGGTATGGATATGGGCAGAATTTTACTCGATTTATCCGATGAGGTGATTAAGCGACTTGATGATCT
CAAAGTGCAGCGCAATCTTCCTCGCGCAGAGCTATTACGAGAAGCTGTAGATCAATATCTGGAAAATCAATCGAAAACGA
CAATATCCAGTGCTTTTGGTATCTGGCAAGGGTGTGAGGAAGATGGTGTCGAATATCAGCGTAAGCTGCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References