Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 477211..477782 Replicon chromosome
Accession NZ_CP072884
Organism Enterococcus faecium strain F179

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag J9540_RS02265 Protein ID WP_002286801.1
Coordinates 477441..477782 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag J9540_RS02260 Protein ID WP_002323011.1
Coordinates 477211..477441 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9540_RS02235 (J9540_02240) 472454..473785 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
J9540_RS02240 (J9540_02245) 473807..474433 + 627 WP_002333420.1 cysteine hydrolase -
J9540_RS02245 (J9540_02250) 474616..475197 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
J9540_RS02250 (J9540_02255) 475806..476381 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
J9540_RS02255 (J9540_02260) 476586..476924 - 339 WP_002286804.1 hypothetical protein -
J9540_RS02260 (J9540_02265) 477211..477441 + 231 WP_002323011.1 hypothetical protein Antitoxin
J9540_RS02265 (J9540_02270) 477441..477782 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J9540_RS02270 (J9540_02275) 478632..478817 + 186 WP_002304207.1 hypothetical protein -
J9540_RS02275 (J9540_02280) 479322..479516 + 195 WP_002295146.1 hypothetical protein -
J9540_RS02280 (J9540_02285) 479707..479956 + 250 Protein_454 transposase -
J9540_RS02285 (J9540_02290) 480200..481030 - 831 WP_002286789.1 manganese catalase family protein -
J9540_RS02290 (J9540_02295) 481238..482572 + 1335 WP_002333419.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T199077 WP_002286801.1 NZ_CP072884:477441-477782 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T199077 NZ_CP097170:c4323145-4322795 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATTTACGCGCCCGACTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT199077 WP_002323011.1 NZ_CP072884:477211-477441 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT199077 NZ_CP097170:c4323390-4323139 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References