Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 471423..471994 Replicon chromosome
Accession NZ_CP072881
Organism Enterococcus faecium strain F39

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag J9541_RS02260 Protein ID WP_002302307.1
Coordinates 471653..471994 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag J9541_RS02255 Protein ID WP_002323011.1
Coordinates 471423..471653 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9541_RS02230 (J9541_02230) 466777..468108 + 1332 WP_002300334.1 FAD-containing oxidoreductase -
J9541_RS02235 (J9541_02235) 468130..468756 + 627 WP_002300333.1 cysteine hydrolase -
J9541_RS02240 (J9541_02240) 468949..469530 + 582 WP_002300332.1 TetR/AcrR family transcriptional regulator -
J9541_RS02245 (J9541_02245) 470017..470592 + 576 WP_002302305.1 SOS response-associated peptidase family protein -
J9541_RS02250 (J9541_02250) 470797..471135 - 339 WP_002286804.1 hypothetical protein -
J9541_RS02255 (J9541_02255) 471423..471653 + 231 WP_002323011.1 hypothetical protein Antitoxin
J9541_RS02260 (J9541_02260) 471653..471994 + 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J9541_RS02270 (J9541_02270) 474460..476964 + 2505 WP_002317782.1 prealbumin-like fold domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T199073 WP_002302307.1 NZ_CP072881:471653-471994 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T199073 NZ_CP097170:3758190-3758408 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT199073 WP_002323011.1 NZ_CP072881:471423-471653 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT199073 NZ_CP097170:3757790-3758164 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References