Detailed information of TA system TA199071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 226386..227494
• Replicon: plasmid pF88_1
• Accession: NZ_CP072879
• Organism: Enterococcus faecium strain F88

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A3S8RBH6
• Locus tag: J9539_RS13890
• Protein ID: WP_071661597.1
• Coordinates: 226625..227494 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: J9539_RS13885
• Protein ID: WP_000205227.1
• Coordinates: 226386..226610 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9539_RS13870 (J9539_13870)	222017..223501	+	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-
J9539_RS13875 (J9539_13875)	223555..224358	+	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
J9539_RS13880 (J9539_13880)	224667..225968	-	1302	Protein_249	ISL3 family transposase	-
J9539_RS13885 (J9539_13885)	226386..226610	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
J9539_RS13890 (J9539_13890)	226625..227494	+	870	WP_071661597.1	nucleotidyltransferase domain-containing protein	Toxin
J9539_RS13895 (J9539_13895)	227475..228209	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
J9539_RS13900 (J9539_13900)	228242..228352	+	111	Protein_253	aminoglycoside 6-adenylyltransferase	-
J9539_RS13905 (J9539_13905)	228371..228763	+	393	WP_000393259.1	GNAT family N-acetyltransferase	-
J9539_RS13910 (J9539_13910)	228839..230017	-	1179	WP_000997695.1	IS256-like element ISEf1 family transposase	-
J9539_RS13915 (J9539_13915)	230110..231549	+	1440	WP_255322375.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32902.55 Da
• Isoelectric Point: 4.9770

>T199071 WP_071661597.1 NZ_CP072879:226625-227494 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T199071 NZ_CP097170:3328697-3329083 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAAGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAGGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT199071 WP_000205227.1 NZ_CP072879:226386-226610 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT199071 NZ_CP097170:3329073-3329402 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A3S8RBH6