Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 492507..493078 Replicon chromosome
Accession NZ_CP072878
Organism Enterococcus faecium strain F88

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag J9539_RS02330 Protein ID WP_002286801.1
Coordinates 492737..493078 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag J9539_RS02325 Protein ID WP_002323011.1
Coordinates 492507..492737 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9539_RS02300 (J9539_02300) 487747..489078 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
J9539_RS02305 (J9539_02305) 489100..489726 + 627 WP_002286816.1 cysteine hydrolase -
J9539_RS02310 (J9539_02310) 489909..490490 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
J9539_RS02315 (J9539_02315) 491102..491677 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
J9539_RS02320 (J9539_02320) 491882..492220 - 339 WP_002286804.1 hypothetical protein -
J9539_RS02325 (J9539_02325) 492507..492737 + 231 WP_002323011.1 hypothetical protein Antitoxin
J9539_RS02330 (J9539_02330) 492737..493078 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J9539_RS02335 (J9539_02335) 493928..494113 + 186 WP_002304207.1 hypothetical protein -
J9539_RS02340 (J9539_02340) 494618..494812 + 195 WP_002295146.1 hypothetical protein -
J9539_RS02345 (J9539_02345) 495003..495252 + 250 Protein_464 transposase -
J9539_RS02350 (J9539_02350) 495496..496326 - 831 WP_002286789.1 manganese catalase family protein -
J9539_RS02355 (J9539_02355) 496534..497868 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T199070 WP_002286801.1 NZ_CP072878:492737-493078 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T199070 NZ_CP097170:c2863681-2863579 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT199070 WP_002323011.1 NZ_CP072878:492507-492737 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT199070 NZ_CP097170:2863541-2863812 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References