199065

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1434505..1435121 Replicon chromosome
Accession NZ_CP072873
Organism Pseudomonas fluorescens strain JNU01

Toxin (Protein)


Gene name hicA Uniprot ID A0A2T4FRB4
Locus tag J9321_RS06265 Protein ID WP_065899432.1
Coordinates 1434505..1434687 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2T4FR72
Locus tag J9321_RS06270 Protein ID WP_065899433.1
Coordinates 1434711..1435121 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9321_RS06240 (J9321_06240) 1430045..1430569 + 525 WP_120447833.1 DUF3833 domain-containing protein -
J9321_RS06245 (J9321_06245) 1430590..1430895 + 306 WP_120447832.1 DUF6482 family protein -
J9321_RS06250 (J9321_06250) 1430991..1432187 - 1197 WP_120447831.1 MFS transporter -
J9321_RS06255 (J9321_06255) 1432184..1433347 - 1164 WP_120447830.1 MFS transporter -
J9321_RS06260 (J9321_06260) 1433443..1434336 + 894 WP_065899431.1 LysR family transcriptional regulator -
J9321_RS06265 (J9321_06265) 1434505..1434687 + 183 WP_065899432.1 type II toxin-antitoxin system HicA family toxin Toxin
J9321_RS06270 (J9321_06270) 1434711..1435121 + 411 WP_065899433.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J9321_RS06275 (J9321_06275) 1435212..1435712 + 501 WP_065899434.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
J9321_RS06280 (J9321_06280) 1435671..1436495 - 825 WP_120447829.1 GNAT family N-acetyltransferase -
J9321_RS06285 (J9321_06285) 1436675..1437100 + 426 WP_065899436.1 low affinity iron permease family protein -
J9321_RS06290 (J9321_06290) 1437154..1437681 - 528 WP_016975135.1 DUF4142 domain-containing protein -
J9321_RS06295 (J9321_06295) 1437800..1438441 - 642 WP_106804881.1 LysE family translocator -
J9321_RS06300 (J9321_06300) 1438486..1438896 - 411 WP_120447828.1 fosfomycin resistance glutathione transferase -
J9321_RS06305 (J9321_06305) 1438909..1439835 - 927 WP_120447827.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(11-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6850.06 Da        Isoelectric Point: 10.8600

>T199065 WP_065899432.1 NZ_CP072873:1434505-1434687 [Pseudomonas fluorescens]
VDSRYLMGHIVADGWYLVRIRGSHHHFRHPTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Download         Length: 183 bp

>T199065 NZ_CP097170:c2147575-2147081 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14770.68 Da        Isoelectric Point: 4.4796

>AT199065 WP_065899433.1 NZ_CP072873:1434711-1435121 [Pseudomonas fluorescens]
MLYPIAISTGDQDHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPSAQKVTVHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQEH

Download         Length: 411 bp

>AT199065 NZ_CP097170:c2147865-2147572 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2T4FRB4

Antitoxin

Source ID Structure
AlphaFold DB A0A2T4FR72

References