Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rlegTA/AbiEii-AbiEi
Location 268637..270116 Replicon plasmid p4
Accession NZ_CP072869
Organism Rhizobium sp. NLR16a

Toxin (Protein)


Gene name rlegT Uniprot ID -
Locus tag J7U39_RS28250 Protein ID WP_210633088.1
Coordinates 268637..269506 (-) Length 290 a.a.

Antitoxin (Protein)


Gene name rlegA Uniprot ID -
Locus tag J7U39_RS28255 Protein ID WP_127431473.1
Coordinates 269499..270116 (-) Length 206 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J7U39_RS28235 (J7U39_28235) 266069..266635 + 567 WP_210633085.1 conjugative transfer signal peptidase TraF -
J7U39_RS28240 (J7U39_28240) 266625..267788 + 1164 WP_210633086.1 conjugal transfer protein TraB -
J7U39_RS28245 (J7U39_28245) 267805..268410 + 606 WP_210633087.1 TraH family protein -
J7U39_RS28250 (J7U39_28250) 268637..269506 - 870 WP_210633088.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
J7U39_RS28255 (J7U39_28255) 269499..270116 - 618 WP_127431473.1 type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein Antitoxin
J7U39_RS32030 270163..270374 - 212 Protein_257 hypothetical protein -
J7U39_RS28260 (J7U39_28260) 270464..270706 - 243 WP_087002841.1 helix-turn-helix transcriptional regulator -
J7U39_RS28265 (J7U39_28265) 270930..271253 + 324 WP_210633218.1 transcriptional repressor TraM -
J7U39_RS28270 (J7U39_28270) 271257..271961 - 705 WP_210633089.1 autoinducer binding domain-containing protein -
J7U39_RS28275 (J7U39_28275) 272273..273571 - 1299 WP_210633090.1 IncP-type conjugal transfer protein TrbI -
J7U39_RS28280 (J7U39_28280) 273583..274023 - 441 WP_210633091.1 conjugal transfer protein TrbH -
J7U39_RS28285 (J7U39_28285) 274027..274839 - 813 WP_210633092.1 P-type conjugative transfer protein TrbG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..404135 404135


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-263)

Antitoxin

Transcriptional regulator, AbiEi antitoxin

(15-56)


Sequences

Toxin        


Download         Length: 290 a.a.        Molecular weight: 32539.33 Da        Isoelectric Point: 5.0710

>T199061 WP_210633088.1 NZ_CP072869:c269506-268637 [Rhizobium sp. NLR16a]
MAKEIRNVGASVRARLLQLAKASGQSFDLVLTRFALERLLFRLSQSPHADRFVLKGAMLMMSWFDNPHRGTRDLDLLGFG
DPSPDPMLETFREILAQEADDGVTFDADTLHVDRIREALDYGGLRLRVIASISGARINLTIDIGFGDALEPGVEVLDYPS
MLDFPMPRLRAYARETVIAEKFQAMVMLGRVNSRMKDFYDIWILSRSFDFSDDRLARAIAATFDRRETPIPIDLPDALTD
AFAKDQQKQRQWRAFIEGVAHNPGDLIDVIAQIATFLMPHAVAAARLDE

Download         Length: 870 bp

>T199061 NZ_CP097170:c894875-894498 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGTGCTGCCCGTCTCCCGTCACCATCTGGCATACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACCGAACAGTAAATAACATTACTCTGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 206 a.a.        Molecular weight: 22389.86 Da        Isoelectric Point: 10.2141

>AT199061 WP_127431473.1 NZ_CP072869:c270116-269499 [Rhizobium sp. NLR16a]
MPSSVTQRQIARTVLTERGIARFVELRNAGVTAATMSRMERDGEVLRLARGLYQLPDAQLDASHSLAEVAKRAPKAVICL
VSALAFHSLTDQLPGQIWLAIGRKDWPPKLEAPAVRIVRFTDSLLNDSVETHIIEGVPVRVFGIAKTIADCFRYRNKIGL
SVAIEGLQEALRQRRATPGEIANQAERGGVGSVIRPYLEALTANG

Download         Length: 618 bp

>AT199061 NZ_CP097170:c895296-894922 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGGCCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References