Detailed information of TA system TA199060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 47292..47947
• Replicon: plasmid p4
• Accession: NZ_CP072869
• Organism: Rhizobium sp. NLR16a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A432N9U1
• Locus tag: J7U39_RS27230
• Protein ID: WP_018496488.1
• Coordinates: 47292..47711 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: J7U39_RS27235
• Protein ID: WP_127431704.1
• Coordinates: 47711..47947 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7U39_RS31955	43550..43846	-	297	WP_247241811.1	iron-containing alcohol dehydrogenase	-
J7U39_RS27210 (J7U39_27210)	44097..45362	-	1266	WP_210633163.1	plasmid partitioning protein RepA	-
J7U39_RS27215 (J7U39_27215)	45635..45912	+	278	Protein_41	substrate-binding domain-containing protein	-
J7U39_RS31965	46325..46793	-	469	Protein_42	ABC transporter permease subunit	-
J7U39_RS27225 (J7U39_27225)	46818..47143	+	326	Protein_43	DDE-type integrase/transposase/recombinase	-
J7U39_RS27230 (J7U39_27230)	47292..47711	-	420	WP_018496488.1	type II toxin-antitoxin system VapC family toxin	Toxin
J7U39_RS27235 (J7U39_27235)	47711..47947	-	237	WP_127431704.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J7U39_RS27240 (J7U39_27240)	49039..49509	+	471	WP_210633164.1	TlpA disulfide reductase family protein	-
J7U39_RS27245 (J7U39_27245)	49787..50482	+	696	WP_210633165.1	response regulator transcription factor	-
J7U39_RS27250 (J7U39_27250)	50723..50965	+	243	WP_210633166.1	hypothetical protein	-
J7U39_RS27255 (J7U39_27255)	50980..51099	-	120	Protein_49	transposase	-
J7U39_RS31970	51179..51323	-	145	Protein_50	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..404135	404135
	-	flank	IS/Tn	-	-	50977..51099	122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-117)

Antitoxin

• SpoVT-AbrB domain (7-44)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15616.86 Da
• Isoelectric Point: 4.9496

>T199060 WP_018496488.1 NZ_CP072869:c47711-47292 [Rhizobium sp. NLR16a]
MARYMLDTNMCIYLMKNQPEQVARRFASCYVGDVVMSAITFAELEYGVSASDDPARELDNLTALAELITVEPFGIAAARA
YGPVRMATRDRKKDHLDKLIASHAIALDTVLVTNNTRDFLAYPGLKLENWLDAERRETN

Nucleotide

• Length: 420 bp

>T199060 NZ_CP097170:802877-803173 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9065.18 Da
• Isoelectric Point: 5.6514

>AT199060 WP_127431704.1 NZ_CP072869:c47947-47711 [Rhizobium sp. NLR16a]
MHTTKAFKNGNSQAVRIPAELAFERTDVELEIERIGDELRIRPVHRSLAGVLDVFARFSPDFMRDGRESHEQEERDKI

Nucleotide

• Length: 237 bp

>AT199060 NZ_CP097170:803175-803570 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: A0A432N9U1