Detailed information of TA system TA199059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 288493..289073
• Replicon: plasmid p2
• Accession: NZ_CP072867
• Organism: Rhizobium sp. NLR16a

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J7U39_RS23575
• Protein ID: WP_210632233.1
• Coordinates: 288493..288876 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J7U39_RS23580
• Protein ID: WP_210632655.1
• Coordinates: 288873..289073 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J7U39_RS23550 (J7U39_23550)	284517..286025	-	1509	WP_210632229.1	alpha-N-arabinofuranosidase	-
J7U39_RS23555 (J7U39_23555)	286237..287163	-	927	WP_210632230.1	helix-turn-helix domain-containing protein	-
J7U39_RS23560 (J7U39_23560)	287269..287690	-	422	Protein_257	type II toxin-antitoxin system VapC family toxin	-
J7U39_RS23565 (J7U39_23565)	287687..287935	-	249	WP_210632231.1	plasmid stabilization protein	-
J7U39_RS23570 (J7U39_23570)	288166..288522	+	357	WP_210632232.1	hypothetical protein	-
J7U39_RS23575 (J7U39_23575)	288493..288876	-	384	WP_210632233.1	type II toxin-antitoxin system VapC family toxin	Toxin
J7U39_RS23580 (J7U39_23580)	288873..289073	-	201	WP_210632655.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J7U39_RS23585 (J7U39_23585)	289313..289657	-	345	Protein_262	cytochrome-c peroxidase	-
J7U39_RS23590 (J7U39_23590)	289664..290369	+	706	Protein_263	acid phosphatase	-
J7U39_RS23595 (J7U39_23595)	290425..291183	-	759	WP_210632234.1	IclR family transcriptional regulator	-
J7U39_RS23600 (J7U39_23600)	291316..292023	+	708	WP_210632235.1	3-oxoacid CoA-transferase subunit A	-
J7U39_RS23605 (J7U39_23605)	292020..292709	+	690	WP_210632236.1	CoA transferase subunit B	-
J7U39_RS23610 (J7U39_23610)	292720..293925	+	1206	WP_210632237.1	3-oxoadipyl-CoA thiolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..519704	519704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14109.28 Da
• Isoelectric Point: 7.4758

>T199059 WP_210632233.1 NZ_CP072867:c288876-288493 [Rhizobium sp. NLR16a]
VILADTSIWIDHFRHADPELRKIIEDDRLLCHPAVIGELALGSLRNRGSVIAFLAAQREVAVATHDEVMVMIDRHNIFSM
GIGYTDAHLLASVLLDRRAALWTRDKRLQGAAGKAGVSLYTPVDRRS

Nucleotide

• Length: 384 bp

>T199059 NZ_CP097170:c690754-690290 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7063.27 Da
• Isoelectric Point: 11.2292

>AT199059 WP_210632655.1 NZ_CP072867:c289073-288873 [Rhizobium sp. NLR16a]
MRSTINLDDALMQKAKALTGTKETAALVRQALETLVRVESGKRLIALGGTMPEAEAAPRRRSAVAK

Nucleotide

• Length: 201 bp

>AT199059 NZ_CP097170:c691089-690754 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA