Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4074550..4075126 Replicon chromosome
Accession NZ_CP072858
Organism Escherichia coli strain 184/2aE

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag J9158_RS19660 Protein ID WP_001297643.1
Coordinates 4074839..4075126 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag J9158_RS19655 Protein ID WP_000063156.1
Coordinates 4074550..4074852 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9158_RS19640 (4071146) 4071146..4073296 + 2151 WP_001446922.1 pyruvate/proton symporter BtsT -
J9158_RS19645 (4073346) 4073346..4073549 + 204 WP_000467859.1 YbdD/YjiX family protein -
J9158_RS19650 (4073560) 4073560..4074516 + 957 WP_001297640.1 GTPase -
J9158_RS19655 (4074550) 4074550..4074852 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
J9158_RS19660 (4074839) 4074839..4075126 - 288 WP_001297643.1 BrnT family toxin Toxin
J9158_RS19665 (4075325) 4075325..4076022 - 698 Protein_3871 IS1 family transposase -
J9158_RS19670 (4076079) 4076079..4076174 + 96 Protein_3872 dienelactone hydrolase family protein -
J9158_RS19675 (4076320) 4076320..4077552 + 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
J9158_RS19680 (4077593) 4077593..4078873 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4075325..4075534 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T199047 WP_001297643.1 NZ_CP072858:c4075126-4074839 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T199047 NZ_CP097128:2659354-2659740 [Conexibacter sp. DBS9H8]
GTGCCTGAAAGCGTTGTACTCGATTCGTCGGCGATGGTCGAGGCGTTGATGGGCACACAGCTGGGAAGAGCGACGCGCGC
CCGTCTTCGCGGGTGTGAGCTGCATGCACCGGCGCACTTCGACGCTGAGGTTCTCTCCGCGGTGGGCCGGTTGCATCGCG
CAGGCGAGCTCGACGCGACGATCGTTTCGACATCACTGAACGAGCTTGCTGTCGCGCCGATCGTCCGACATCCCCTTGCC
GGACTCCTGATTGGAGCATGGGCCGCCCGTGACCGGCTGCGGCTCGTCGACGCTCTCTATGTCGAGTTGAGCACCGTACT
CGGCGCCGTGCTCGTGACGACGGACGCGCGCCTCGCCCGGAGCACGGCGGCGGACCTCGTCAGCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT199047 WP_000063156.1 NZ_CP072858:c4074852-4074550 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT199047 NZ_CP097128:2659140-2659361 [Conexibacter sp. DBS9H8]
ATGGCTCGGGTCAACGTCTATCTTCCGGACGAGCTCGCAGAGCGCGCACGAGCGGCGGGGGTGAATATCTCGGGCGTCAC
CCAGGATGCACTCCGTGGCGCGCTCGCCAGCGTCGACACCGACCGTTGGCTTGATCGGCTCGAGCGGCTGCCGGCCCGCG
ACGTCGCTCACGAGCGCGTGATCTGCGCCCTCGATGAGGCGCGTGAGGACTTCGGTGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References