Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3478355..3478973 Replicon chromosome
Accession NZ_CP072858
Organism Escherichia coli strain 184/2aE

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J9158_RS16765 Protein ID WP_001291435.1
Coordinates 3478755..3478973 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J9158_RS16760 Protein ID WP_000344800.1
Coordinates 3478355..3478729 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9158_RS16750 (3473444) 3473444..3474637 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J9158_RS16755 (3474660) 3474660..3477809 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
J9158_RS16760 (3478355) 3478355..3478729 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J9158_RS16765 (3478755) 3478755..3478973 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J9158_RS16770 (3479145) 3479145..3479696 + 552 WP_000102536.1 maltose O-acetyltransferase -
J9158_RS16775 (3479812) 3479812..3480282 + 471 WP_000136192.1 YlaC family protein -
J9158_RS16780 (3480446) 3480446..3481996 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J9158_RS16785 (3482038) 3482038..3482391 - 354 WP_000878140.1 DUF1428 domain-containing protein -
J9158_RS16795 (3482770) 3482770..3483081 + 312 WP_000409911.1 MGMT family protein -
J9158_RS16800 (3483112) 3483112..3483684 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T199044 WP_001291435.1 NZ_CP072858:3478755-3478973 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T199044 NZ_CP097128:217017-217289 [Conexibacter sp. DBS9H8]
TTGAGCCAGAAAGCCTGGGGCCTTGAGATCGCGGGTCCGGCTGCCCGCGACGTTGAGCGGCTTCCAGCAAAATACGCTGC
CGCGATTCTCACTCTCCTGCCCGCGATCGCAGAGCACCCGCATCGACTTGGCAAGCCACTGCGGCGAGAGCTTGACGGAG
TTTGGTCAGCCCGCCGTGGCCCTTACCGCGTGCTCTACGAGATCGACGAGCAGCGGCACGTCGTGACCGGCGTCGCGGTC
GCCCATCGATCGGACGCCTACCGGCCGAGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT199044 WP_000344800.1 NZ_CP072858:3478355-3478729 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT199044 NZ_CP097128:216748-217020 [Conexibacter sp. DBS9H8]
ATGACCGAGACGCTGCCGCTCGCAACCGTCAAGGCACAGTTCTCCGCAATCGTGGACCGCGTCGAGAGCGAGCATGATCG
CGTGGTCGTCACCCGCAACGGCCGCGCCGCCGCAGTGTTGATCAGCACCGATGATCTTGAAAGTCTGGAAGAGACTGTCG
CGGTCCTGTCAGACCGAGTGCTGATGGCGCGGGTCAATGAGGGACGGCGAGCCGCACAGAGTGGCGACACGATCTCCCTG
GACGAGCTCAAAGCCCGTCTCGCCAGCTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References