Detailed information of TA system TA199040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2444604..2445242
• Replicon: chromosome
• Accession: NZ_CP072858
• Organism: Escherichia coli strain 184/2aE

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: J9158_RS11745
• Protein ID: WP_001447010.1
• Coordinates: 2445066..2445242 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J9158_RS11740
• Protein ID: WP_001270286.1
• Coordinates: 2444604..2445020 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9158_RS11720 (2439767)	2439767..2440708	-	942	WP_001251304.1	ABC transporter permease	-
J9158_RS11725 (2440709)	2440709..2441722	-	1014	WP_000220395.1	ABC transporter ATP-binding protein	-
J9158_RS11730 (2441740)	2441740..2442885	-	1146	WP_000047428.1	ABC transporter substrate-binding protein	-
J9158_RS11735 (2443119)	2443119..2444525	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
J9158_RS11740 (2444604)	2444604..2445020	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J9158_RS11745 (2445066)	2445066..2445242	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J9158_RS11750 (2445464)	2445464..2445694	+	231	WP_000494244.1	YncJ family protein	-
J9158_RS11755 (2445786)	2445786..2447747	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J9158_RS11760 (2447820)	2447820..2448356	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
J9158_RS11765 (2448448)	2448448..2449623	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T199040 WP_001447010.1 NZ_CP072858:c2445242-2445066 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T199040 NZ_CP097127:c2167446-2167204 [Conexibacter sp. S30A1]
ATGCCGCCAGCTCGCCGCGAGTTCGACAGGCTCCCAATCTCGGTCGCAGCCGCGGTTCTGGAAACGCTCGACGCGATCGC
CGAAAACCCTCGACGGTTGGGTAAGCCGCTGATGTTCGAACACCAGGGACGGTTCTCGGCCAGGCGCGGGCCCTATCGGA
TCATCTACGAGCTCGATGAACCTCAACACCTCGTTCGCGTAGTCGCGATTGGCCATCGACGCGACGTTTACCGCCAGCGC
TGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT199040 WP_001270286.1 NZ_CP072858:c2445020-2444604 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT199040 NZ_CP097127:c2167763-2167473 [Conexibacter sp. S30A1]
ATGGCTGAGACGATGTCGCTTGCGAGCGCGAAGGCCAGGTTCTCCGAGTTGGTCGCTCGCGTGGAGCGCCAGCAGGATCG
CGTGATCGTCACGCGCAACGGCAAGCCGGCCGCCGTCGTGATCAGCGTGGACGACCTCGAGAGCCTCGAGGAAACGCTGG
CCGTTATGTCAGACGCCGGCCTGGTGGCTCAGATCCAGGAGAGCCTGTCGACGGCAGCCGCAGGCGACGCAGGGGTCGGC
CTTGACGATCTGAGGGCTGATCTGCAGTCGCGTCGAGCGACCAAGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8