Detailed information of TA system TA199039

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2356628..2358216
• Replicon: chromosome
• Accession: NZ_CP072858
• Organism: Escherichia coli strain 184/2aE

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: J9158_RS11370
• Protein ID: WP_001125439.1
• Coordinates: 2356894..2358216 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J9158_RS11365
• Protein ID: WP_001301023.1
• Coordinates: 2356628..2356894 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9158_RS11360 (2352473)	2352473..2356405	+	3933	Protein_2236	ECSE_1600 family autotransporter	-
J9158_RS11365 (2356628)	2356628..2356894	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J9158_RS11370 (2356894)	2356894..2358216	+	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
J9158_RS11375 (2358528)	2358528..2358736	+	209	Protein_2239	AraC family transcriptional regulator	-
J9158_RS11380 (2358991)	2358991..2360217	+	1227	Protein_2240	fimbria/pilus outer membrane usher protein	-
J9158_RS11385 (2360231)	2360231..2360761	+	531	WP_000876764.1	fimbrial protein	-
J9158_RS11390 (2360774)	2360774..2361277	+	504	WP_000825452.1	fimbrial protein	-
J9158_RS11395 (2361336)	2361336..2362250	+	915	WP_000520662.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2349649..2369437	19788

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T199039 WP_001125439.1 NZ_CP072858:2356894-2358216 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T199039 NZ_CP097127:c1979304-1978888 [Conexibacter sp. S30A1]
GTGGGCCTGCTCGACGTCAACGCGCTCGTCGCGCTGGCTTGGGACTCGCACGTGCACCACGCGTCGGTGCGCTCCTGGAT
GCGCTCGAGCGGCCGCGCGGGATGGGCGACCTGCCCGGTTACCGAGAGCGGCTTCGTGCGCGTCTCCTCGAACCCGAAGG
TGCTGCCAAGTCCGATCGGAGTCGCAGCGGCGCGCGCGGTGCTGGTCGCGCTTCGTGCCGCTGAGGGACACCGCTTCCTG
GGTGATGAGGTGTCTGAGTGCGATCCCGACGTGCCGGAGGTCCACGGCTATCGACAGGTGACCGATGCACACCTGCTCAC
GTTGGCGCGCCGTCACGGGGTCCGCCTTGTGACATTCGACAGCGGGATCGCCGCTCTCGCCCAGGGCGGCGACGTGGAGC
TGCTCACAACCATCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT199039 WP_001301023.1 NZ_CP072858:2356628-2356894 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT199039 NZ_CP097127:c1979533-1979312 [Conexibacter sp. S30A1]
ATGAGAACCACGCTCGACATCGACGACGACGTGGTTACGGCAGCGCGTGAGCTGGCTGCGATCGAGAAGCGGTCGCTCGG
CACGGTGATCTCGAGGTTGGCGCGGCGGGGGCTGACGCCCGCCGCGGTCGATTCCGACGAGGGCTTGCCCGTGATTCGGG
TCCCGGCGGGGACGCCGCCGATCACTTCCGAGATGGTGCGACGCGCCCTCGACGAGGACTGA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU