Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 1808896..1809727 | Replicon | chromosome |
| Accession | NZ_CP072858 | ||
| Organism | Escherichia coli strain 184/2aE | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | S1P968 |
| Locus tag | J9158_RS08670 | Protein ID | WP_000854814.1 |
| Coordinates | 1808896..1809270 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | B3Y195 |
| Locus tag | J9158_RS08675 | Protein ID | WP_001285585.1 |
| Coordinates | 1809359..1809727 (-) | Length | 123 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J9158_RS08630 (1804301) | 1804301..1805521 | + | 1221 | WP_000343760.1 | ISL3-like element ISKox3 family transposase | - |
| J9158_RS08635 (1805578) | 1805578..1806051 | + | 474 | WP_032224276.1 | DNA gyrase inhibitor SbmC | - |
| J9158_RS08640 (1806248) | 1806248..1807306 | + | 1059 | WP_001200891.1 | FUSC family protein | - |
| J9158_RS08645 (1807478) | 1807478..1807807 | + | 330 | WP_000450409.1 | DUF496 family protein | - |
| J9158_RS08650 (1807908) | 1807908..1808042 | - | 135 | WP_001297944.1 | EutP/PduV family microcompartment system protein | - |
| J9158_RS08655 (1808162) | 1808162..1808290 | + | 129 | Protein_1703 | transposase domain-containing protein | - |
| J9158_RS08660 (1808579) | 1808579..1808659 | - | 81 | Protein_1704 | hypothetical protein | - |
| J9158_RS08665 (1808705) | 1808705..1808899 | - | 195 | WP_000988600.1 | DUF5983 family protein | - |
| J9158_RS08670 (1808896) | 1808896..1809270 | - | 375 | WP_000854814.1 | type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA | Toxin |
| J9158_RS08675 (1809359) | 1809359..1809727 | - | 369 | WP_001285585.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| J9158_RS08680 (1809801) | 1809801..1810022 | - | 222 | WP_000692323.1 | DUF987 domain-containing protein | - |
| J9158_RS08685 (1810085) | 1810085..1810561 | - | 477 | WP_001186726.1 | RadC family protein | - |
| J9158_RS08690 (1810577) | 1810577..1811056 | - | 480 | WP_000860087.1 | antirestriction protein | - |
| J9158_RS08695 (1811138) | 1811138..1811956 | - | 819 | WP_001234629.1 | DUF932 domain-containing protein | - |
| J9158_RS08700 (1812056) | 1812056..1812289 | - | 234 | WP_001119729.1 | DUF905 family protein | - |
| J9158_RS08705 (1812368) | 1812368..1812822 | - | 455 | Protein_1713 | IrmA family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | flank | IS/Tn | - | - | 1804301..1805521 | 1220 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 13898.94 Da Isoelectric Point: 7.8522
>T199030 WP_000854814.1 NZ_CP072858:c1809270-1808896 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T199030 NZ_CP097117:c808082-807804 [Fructilactobacillus cliffordii]
ATGACTAAACAATACAAACCTGCTTTTGAAAATCAATTCAAAAAACATTACAAACAAATGCTAAAACAGCCTAAATATAA
CAAAAATCAATTCAATGAAATATATTACTTATTAATTAACGATGAAGAAATTCCAGCTAAGTTTAACGACCATAACCTCA
TAAATCGAAAACCTGAACGAGAACTTCACATTAAACCAGATTGGCTTTTGATTTATCGGTACGATGGTGATTATATTAAA
TTCATTGATACCGGTAGTCATTCAGACTTGTTTGAATAA
ATGACTAAACAATACAAACCTGCTTTTGAAAATCAATTCAAAAAACATTACAAACAAATGCTAAAACAGCCTAAATATAA
CAAAAATCAATTCAATGAAATATATTACTTATTAATTAACGATGAAGAAATTCCAGCTAAGTTTAACGACCATAACCTCA
TAAATCGAAAACCTGAACGAGAACTTCACATTAAACCAGATTGGCTTTTGATTTATCGGTACGATGGTGATTATATTAAA
TTCATTGATACCGGTAGTCATTCAGACTTGTTTGAATAA
Antitoxin
Download Length: 123 a.a. Molecular weight: 13593.48 Da Isoelectric Point: 6.3139
>AT199030 WP_001285585.1 NZ_CP072858:c1809727-1809359 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN
Download Length: 369 bp
>AT199030 NZ_CP097117:c808326-808075 [Fructilactobacillus cliffordii]
ATGAAAACAAATACAGCACAAATTCATTTTAGAACTAGTACCCTTAATAAGGACGAATTTGAAAAAGTTTTAAAAGAAAA
CGGATTAACTGCTCCAGAAGCGTTTCGTATTTTTATGAAACAAACAATTAACAACAATGGATTACCTTTTGAAGTAAACC
AACCAAATAAACGACTAAAAACAGCAATTAGTAGTGATGATTACGTTAAGTTTGATAATGCTCAGGAAGGTTTAGATTGG
TTAAATGACTAA
ATGAAAACAAATACAGCACAAATTCATTTTAGAACTAGTACCCTTAATAAGGACGAATTTGAAAAAGTTTTAAAAGAAAA
CGGATTAACTGCTCCAGAAGCGTTTCGTATTTTTATGAAACAAACAATTAACAACAATGGATTACCTTTTGAAGTAAACC
AACCAAATAAACGACTAAAAACAGCAATTAGTAGTGATGATTACGTTAAGTTTGATAATGCTCAGGAAGGTTTAGATTGG
TTAAATGACTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829FY50 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LW60 |