Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 860320..860974 Replicon chromosome
Accession NZ_CP072858
Organism Escherichia coli strain 184/2aE

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag J9158_RS04190 Protein ID WP_000244777.1
Coordinates 860567..860974 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J9158_RS04185 Protein ID WP_000354046.1
Coordinates 860320..860586 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9158_RS04160 (855489) 855489..856232 + 744 WP_000951964.1 SDR family oxidoreductase -
J9158_RS04165 (856289) 856289..857722 - 1434 WP_001446971.1 6-phospho-beta-glucosidase BglA -
J9158_RS04170 (857767) 857767..858078 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
J9158_RS04175 (858242) 858242..858901 + 660 WP_000250274.1 hemolysin III family protein -
J9158_RS04180 (859097) 859097..860077 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
J9158_RS04185 (860320) 860320..860586 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J9158_RS04190 (860567) 860567..860974 + 408 WP_000244777.1 protein YgfX Toxin
J9158_RS04195 (861014) 861014..861535 - 522 WP_001055874.1 flavodoxin FldB -
J9158_RS04200 (861647) 861647..862543 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J9158_RS04205 (862568) 862568..863278 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J9158_RS04210 (863284) 863284..865017 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T199028 WP_000244777.1 NZ_CP072858:860567-860974 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T199028 NZ_CP097108:c5713186-5712842 [Pseudomonas bijieensis]
ATGCGTACCCTATTTTTTGAAACGACCACATTCACCGCCACGGTTGGCCATTACCTGACTGATGATGAGTATCGTCTGCT
TCAGTCCTATATGCTGGAGCATCCAGAGGTCGGTGACGTCATGCCACGTACCGGCGGCTTTCGTAAGCTGCGCTGGTTCG
ATGAGCGTCGTGGCAAAGGGAAGCGGGGTGGGTTGCGAGTCATTTACTATTGGCTCATGAACGATCGTCAGTTTTGGATG
TTCGCGATTTATGACAAGGACGAGTTGGTAAACCTGACCTCCGAGCAAGAGAAGACGCTCAAGCGCGCCATAGAAGCTGA
GTTGAAAGTACGAGGTACCTTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT199028 WP_000354046.1 NZ_CP072858:860320-860586 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT199028 NZ_CP097108:c5712845-5712531 [Pseudomonas bijieensis]
ATGAAAAAGCGCGATCTGTTTGCCGAGATGATGCAAGGCGTCGAGGAGATGGCCGCTCACCGCGAGGGCAAGATTACCCT
TCGCCAGATATCGATCGAAGACAAACCGGTTCCCGAGGTGTCGGCCCAGGAAATCGTGGCTTTGCGCGACAAGCTCCACA
TGTCCCAAGCCGTTTTCGCCAAGAGCATCAGGACCAGTCCAGGTACCCTGAGAAATTGGGAACAGGAAAAATCCAAGCCT
AACGCCCAGGCGGCTTTGTTGATCAAACTGGTCGAGAAGTTTCCAGATATGGTCGAACGACTTGGCGCTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References