Detailed information of TA system TA199023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3266676..3267266
• Replicon: chromosome
• Accession: NZ_CP072856
• Organism: Lonsdalea populi strain N-5-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: J8X70_RS14550
• Protein ID: WP_085690046.1
• Coordinates: 3266676..3267008 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: J8X70_RS14555
• Protein ID: WP_085690044.1
• Coordinates: 3267009..3267266 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8X70_RS14535	3262121..3263047	-	927	WP_085690050.1	ParA family protein	-
J8X70_RS14540	3263343..3264611	+	1269	WP_085690048.1	integrase arm-type DNA-binding domain-containing protein	-
J8X70_RS14545	3265034..3265903	-	870	WP_085690073.1	integrase domain-containing protein	-
J8X70_RS14550	3266676..3267008	-	333	WP_085690046.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J8X70_RS14555	3267009..3267266	-	258	WP_085690044.1	antitoxin	Antitoxin
J8X70_RS14560	3267486..3268163	+	678	WP_197669156.1	ParA family protein	-
J8X70_RS14570	3269564..3269812	+	249	WP_139829925.1	hypothetical protein	-
J8X70_RS14575	3269857..3270000	+	144	WP_176222543.1	hypothetical protein	-
J8X70_RS14580	3270224..3271318	-	1095	WP_085687409.1	site-specific tyrosine recombinase XerC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3239432..3274815	35383
	-	flank	IS/Tn	-	-	3269072..3269338	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11768.54 Da
• Isoelectric Point: 10.9269

>T199023 WP_085690046.1 NZ_CP072856:c3267008-3266676 [Lonsdalea populi]
MDRGEIWLVSLDSTAGHEQSGKRPVLIVSPAPFNKITRLPVVVPVTSGGNFARTAGFAVSLDGTGTKTTGVVRCDQPRTI
DMAARNGRRLERIPDSIVNEVLARLGTILT

Nucleotide

• Length: 333 bp

>T199023 NZ_CP097107:c4046320-4046033 [Citrobacter freundii]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCACAAAGTAACCTGAGAAAACACGGTGTCCGGTTTGAGGATGCGGT
GCTGGTGTTTGACGATCCCCAACATCTTTCGCGGCAGGATCGGCATGAGAACGGTGAGTATCGCTGGCAGACCATCGGCC
TCGTTCATGGCATTGTGGTTATTCTGGTTGCCCATAGCGTTAGGTTTGAAAGTGGCTTTGAAGTCATTCGTATCATCAGT
GCCAGAAAAGCAGACAGTAAGGAGAGGAGTCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.60 Da
• Isoelectric Point: 4.5368

>AT199023 WP_085690044.1 NZ_CP072856:c3267266-3267009 [Lonsdalea populi]
MYTTRLKKVGGSVMLAVPPAVLKTLDLSPDSEVGVTVDNGCLIIEPQKRPHYSLAELLAQCDPHAQVSDEDREWIDAPAT
GKEIL

Nucleotide

• Length: 258 bp

>AT199023 NZ_CP097107:c4046046-4045744 [Citrobacter freundii]
ATGAACATGGTTAAGCATAAACGCGGCAGCATATCTGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAAGCGCTGGCGAA
TAAACCGGACGATGATATCGATTACAGTGACATTCCCGTGACCGAAGATAAGCAGTGGTCAGAGGCCACACGCGGTAAGT
TTTTCCGTCCGTTAAAAACGCAGGCCTCGGTGCGTATTGATGCCGATGTGATGGAGTGGCTGAAACGTCCAGGAAAAGGA
TATCAGACGCGCCTTAATGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG