Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2830890..2831443 Replicon chromosome
Accession NZ_CP072856
Organism Lonsdalea populi strain N-5-1

Toxin (Protein)


Gene name ccdB Uniprot ID A0A1V2R684
Locus tag J8X70_RS12705 Protein ID WP_039360030.1
Coordinates 2830890..2831204 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A3S1FM98
Locus tag J8X70_RS12710 Protein ID WP_029729596.1
Coordinates 2831207..2831443 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8X70_RS12685 2826199..2827215 + 1017 WP_085689715.1 P-type DNA transfer ATPase VirB11 -
J8X70_RS12690 2827259..2827561 + 303 WP_085689717.1 conjugal transfer protein -
J8X70_RS12695 2828359..2830095 + 1737 WP_085689719.1 type IV secretory system conjugative DNA transfer family protein -
J8X70_RS12700 2830104..2830856 + 753 WP_085689721.1 MobC family replication-relaxation protein -
J8X70_RS12705 2830890..2831204 - 315 WP_039360030.1 CcdB family protein Toxin
J8X70_RS12710 2831207..2831443 - 237 WP_029729596.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J8X70_RS12715 2831763..2832016 - 254 Protein_2466 zincin-like metallopeptidase domain-containing protein -
J8X70_RS12720 2832429..2833514 + 1086 WP_085689723.1 SIR2 family protein -
J8X70_RS12725 2833516..2834889 + 1374 WP_232100004.1 ATP-binding protein -
J8X70_RS12735 2836084..2836416 + 333 Protein_2470 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2805452..2839923 34471


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(9-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11535.48 Da        Isoelectric Point: 7.9859

>T199022 WP_039360030.1 NZ_CP072856:c2831204-2830890 [Lonsdalea populi]
MQFTVYGNTGKSVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPAGRRPDRLVPVVRLTDGKEYAVMTHELASIPVQALG
AVFCDASQYRTQVKAAIDFLIDGF

Download         Length: 315 bp

>T199022 NZ_CP097107:3670945-3671286 [Citrobacter freundii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATAGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8927.96 Da        Isoelectric Point: 6.4612

>AT199022 WP_029729596.1 NZ_CP072856:c2831443-2831207 [Lonsdalea populi]
MTAKQRNTQSVTMTVERALLVRAREAGINLSATLTTALDAELRSHEAKKWQEENREALAALNRFHDEQGCFSDEYRTF

Download         Length: 237 bp

>AT199022 NZ_CP097107:3670607-3670924 [Citrobacter freundii]
ATGAGCAAAATCACATGGGGTCTGCAGCGTGATATCACGCCACGTCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATGACCGGCAAGTTCAGTGACATCGAATACCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACTGGTGAACTCAGCACCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1V2R684

Antitoxin

Source ID Structure
AlphaFold DB A0A3S1FM98

References