Detailed information of TA system TA199021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1529484..1530108
• Replicon: chromosome
• Accession: NZ_CP072856
• Organism: Lonsdalea populi strain N-5-1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: E0SD87
• Locus tag: J8X70_RS06925
• Protein ID: WP_009114000.1
• Coordinates: 1529484..1529687 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: J8X70_RS06930
• Protein ID: WP_085689888.1
• Coordinates: 1529740..1530108 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8X70_RS06895	1525115..1525453	+	339	WP_009114005.1	P-II family nitrogen regulator	-
J8X70_RS06900	1525494..1526786	+	1293	WP_085689883.1	ammonium transporter AmtB	-
J8X70_RS06905	1526891..1527754	-	864	WP_085689915.1	acyl-CoA thioesterase II	-
J8X70_RS06910	1527964..1528536	+	573	WP_085689885.1	YbaY family lipoprotein	-
J8X70_RS06915	1528557..1528877	-	321	WP_085689887.1	MGMT family protein	-
J8X70_RS06925	1529484..1529687	-	204	WP_009114000.1	HHA domain-containing protein	Toxin
J8X70_RS06930	1529740..1530108	-	369	WP_085689888.1	Hha toxicity modulator TomB	Antitoxin
J8X70_RS06935	1530718..1530858	-	141	Protein_1337	50S ribosomal protein L36	-
J8X70_RS06940	1531332..1531560	-	229	Protein_1338	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8133.51 Da
• Isoelectric Point: 8.8500

>T199021 WP_009114000.1 NZ_CP072856:c1529687-1529484 [Lonsdalea populi]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFYSAADHRLAELTMNKLYDKVPTAVWKYVR

Nucleotide

• Length: 204 bp

>T199021 NZ_CP097107:3463622-3463840 [Citrobacter freundii]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATCCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14408.15 Da
• Isoelectric Point: 4.7628

>AT199021 WP_085689888.1 NZ_CP072856:c1530108-1529740 [Lonsdalea populi]
MDEYSPKHYDIAQLRFLCENLRDESIATLGDSNHGWVNDPTSAINLQLNELIEHIAAFIGTYKIKYPDDEELYMRVEKYL
DDTYILFSNYGINDAELRRWQKSKSQLFRMLSEKSNYATVRT

Nucleotide

• Length: 369 bp

>AT199021 NZ_CP097107:3463220-3463594 [Citrobacter freundii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1X3RNQ0