Detailed information of TA system TA199020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1350222..1351942
• Replicon: chromosome
• Accession: NZ_CP072856
• Organism: Lonsdalea populi strain N-5-1

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: J8X70_RS06170
• Protein ID: WP_085686763.1
• Coordinates: 1351286..1351942 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: J8X70_RS06165
• Protein ID: WP_085686765.1
• Coordinates: 1350222..1351289 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8X70_RS06155	1346390..1347106	-	717	WP_085686769.1	SprT family zinc-dependent metalloprotease	-
J8X70_RS06160	1347106..1350201	-	3096	WP_085686767.1	HsdR family type I site-specific deoxyribonuclease	-
J8X70_RS06165	1350222..1351289	-	1068	WP_085686765.1	macro domain-containing protein	Antitoxin
J8X70_RS06170	1351286..1351942	-	657	WP_085686763.1	DUF4433 domain-containing protein	Toxin
J8X70_RS06175	1351955..1353316	-	1362	WP_085686884.1	restriction endonuclease subunit S	-
J8X70_RS06180	1353316..1355760	-	2445	WP_085686761.1	type I restriction-modification system subunit M	-
J8X70_RS06185	1355801..1356040	-	240	WP_085686759.1	transcription factor	-
J8X70_RS06190	1356441..1356902	+	462	WP_085686757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1302205..1358544	56339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 24932.36 Da
• Isoelectric Point: 7.1574

>T199020 WP_085686763.1 NZ_CP072856:c1351942-1351286 [Lonsdalea populi]
MANNYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSPVQAGNWINIGNPELISKRAGHPVPVGTGGVLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSSLRTVAAQNVPFVFTDSHAYYNWANYYTELDNLNQIDWPILQARDFRRDPDD
PGKFERYQAEALIWQHCPIILLGGMVCYNDSVKLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T199020 NZ_CP097107:1907083-1907186 [Citrobacter freundii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40591.86 Da
• Isoelectric Point: 6.5864

>AT199020 WP_085686765.1 NZ_CP072856:c1351289-1350222 [Lonsdalea populi]
MITFTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPANMKAYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRTDSRMEWIEDGLQDLRRFLIEKQVQSIAIPPLGAGNGGLNWPDVRAHIESALGDLQDVEILIYEPTEKYQNVAKST
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIKLHQQENVLKLRFEAHNYGPYAHNLTHLLNALDGNYLK
AEKRIPDSQPLDVIWFNDREKDHVNTYLNSEAKTWLPALEQVSQLIDGFESPFGMELLATVDWLLTCDKCQPTLDSVKEG
LSQWPAGEHWASRKMKLFDDNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT199020 NZ_CP097107:c1907190-1907048 [Citrobacter freundii]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT