Detailed information of TA system TA199018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2970..3712
• Replicon: chromosome
• Accession: NZ_CP072856
• Organism: Lonsdalea populi strain N-5-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: C6C3X4
• Locus tag: J8X70_RS00035
• Protein ID: WP_015855199.1
• Coordinates: 3233..3712 (+)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A3N0G0N1
• Locus tag: J8X70_RS00030
• Protein ID: WP_019843650.1
• Coordinates: 2970..3242 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8X70_RS00010	53..418	+	366	WP_112131668.1	hypothetical protein	-
J8X70_RS00015	428..778	+	351	WP_085690284.1	hypothetical protein	-
J8X70_RS00020	848..1186	-	339	WP_085690282.1	SymE family type I addiction module toxin	-
J8X70_RS00025	1319..2427	+	1109	WP_100059615.1	IS3 family transposase	-
J8X70_RS00030	2970..3242	+	273	WP_019843650.1	DUF1778 domain-containing protein	Antitoxin
J8X70_RS00035	3233..3712	+	480	WP_015855199.1	GNAT family N-acetyltransferase	Toxin
J8X70_RS00040	3983..4198	-	216	WP_051122262.1	helix-turn-helix transcriptional regulator	-
J8X70_RS00045	4294..4560	+	267	WP_085685657.1	helix-turn-helix transcriptional regulator	-
J8X70_RS00050	4784..5863	-	1080	WP_232100170.1	helicase RepA family protein	-
J8X70_RS00055	5987..6829	-	843	Protein_10	primase-helicase zinc-binding domain-containing protein	-
J8X70_RS00060	6822..6998	-	177	WP_226100054.1	hypothetical protein	-
J8X70_RS00065	7080..7358	-	279	Protein_12	ash family protein	-
J8X70_RS00070	7355..7555	-	201	WP_026357784.1	AlpA family transcriptional regulator	-
J8X70_RS00075	7558..8439	-	882	WP_085685663.1	integrase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1597..12884	11287
	-	flank	IS/Tn	-	-	1319..1585	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17538.30 Da
• Isoelectric Point: 9.5367

>T199018 WP_015855199.1 NZ_CP072856:3233-3712 [Lonsdalea populi]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPIPVLVLGRLAVDERYQRMGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVASEIQEHTLLLSLW

Nucleotide

• Length: 480 bp

>T199018 NZ_CP097107:646233-646640 [Citrobacter freundii]
GTGGTCCAGTGGCAATCTGATTTACGCGTATCATGGCGCGCGCAGTGGCTTTCACTGCTAATTCATGGGCTGGTCGCGGT
GTTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTTTGCTCTCTCTGGTGGTATTCGATAGCG
TTCGCAGTCAGCGTCGAATCAACGCCTGTCAGGGTGAGATTCGGCTGTTGATGGATGGACGTCTGCGCTGGCAAGGGCAA
GAGTGGTCTATCGTTAGCGCCCCCTGGATGGTTAAAACAGGCATGATGCTGCGTTTGCGCTCTGATGCCGGAAAACGTCA
ACATTTGTGGCTGGCGGCAGACAGTATGGATGATGCAGAGTGGCGGGATTTACGGCGGTTGATGCTGCAAAAGGCGAAAC
AGAAGTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10388.86 Da
• Isoelectric Point: 4.6702

>AT199018 WP_019843650.1 NZ_CP072856:2970-3242 [Lonsdalea populi]
MRTETKEMPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRVN
ALLNRKSPWE

Nucleotide

• Length: 273 bp

>AT199018 NZ_CP097107:645986-646252 [Citrobacter freundii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGCCGCGGTATGCGCGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGACGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGATT
TATTTAACTGGTTGATGAATCATGGGAAACCAGCTGATGCTGAGCTGGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A2K8QHE3

Antitoxin

• AlphaFold DB: A0A3N0G0N1