199017

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 21794..22365 Replicon plasmid p1
Accession NZ_CP072855
Organism Leptospira interrogans strain UI29382

Toxin (Protein)


Gene name mazF Uniprot ID Q8F5A3
Locus tag J9305_RS19100 Protein ID WP_000240418.1
Coordinates 22024..22365 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M6U553
Locus tag J9305_RS19095 Protein ID WP_000443825.1
Coordinates 21794..22030 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9305_RS19075 (J9305_18990) 18316..18792 + 477 WP_000691822.1 hypothetical protein -
J9305_RS19080 (J9305_18995) 19305..19679 - 375 WP_233683061.1 hypothetical protein -
J9305_RS19085 (J9305_19005) 20028..20354 + 327 WP_000654336.1 hypothetical protein -
J9305_RS19090 (J9305_19010) 20667..21458 + 792 WP_000703027.1 hypothetical protein -
J9305_RS19095 (J9305_19015) 21794..22030 + 237 WP_000443825.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
J9305_RS19100 (J9305_19020) 22024..22365 + 342 WP_000240418.1 endoribonuclease MazF Toxin
J9305_RS19105 (J9305_19025) 22433..22816 - 384 WP_061245003.1 hypothetical protein -
J9305_RS19110 (J9305_19030) 22816..23082 - 267 WP_000844450.1 hypothetical protein -
J9305_RS19115 (J9305_19035) 23143..23394 - 252 WP_061226905.1 hypothetical protein -
J9305_RS19120 (J9305_19040) 23397..24119 - 723 WP_061226906.1 hypothetical protein -
J9305_RS19125 (J9305_19050) 24543..24743 + 201 WP_001206464.1 hypothetical protein -
J9305_RS19130 (J9305_19055) 25266..25727 + 462 WP_000811789.1 hypothetical protein -
J9305_RS19135 (J9305_19060) 25834..26445 + 612 WP_000851077.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..60147 60147


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin

Antitoxin PemI-like

(1-78)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12918.12 Da        Isoelectric Point: 9.9595

>T199017 WP_000240418.1 NZ_CP072855:22024-22365 [Leptospira interrogans]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLIF

Download         Length: 342 bp

>T199017 NZ_CP097107:434846-435187 [Citrobacter freundii]
ATGAAACCTCAATCTGCAGCAACTTCGCGGGCGGTAAAACCCTGCCTGTCACCCGTGGCTATCTGGCAAATTCTACTGAC
ACGCCTGCTGGAACAACACTACGGCCTGACCCTGAACGACACGCCGTTCAGGGATGAATCCGTTATACAGGAACATATCG
ATGCAGGTATCACGCTGGCCAATGCCGTTAACTTTCTGGTGGAAAAATACGAGCTTGTTCGTATCGATCGCAGAAGGTTT
AGTTGGCAGGAACAATCACCTTATCTTCGGGCAGCAGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8728.95 Da        Isoelectric Point: 5.6663

>AT199017 WP_000443825.1 NZ_CP072855:21794-22030 [Leptospira interrogans]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Download         Length: 237 bp

>AT199017 NZ_CP097107:434508-434825 [Citrobacter freundii]
ATGAGCAACATCACTTGGGGCTTGCAGCGCGATATCACGCCGCGCCTGGGAACACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTACCTAGCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCAGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGACCTCCGGTGAACTGAATCCCCTACACGTTCATTGCGTCACCCTGTACCAC
ACCGGTTTTACCTGCGAAGCCGACACCCTCGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTAAGCGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D8SL30

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HC33

References