Detailed information of TA system TA199016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3250389..3251017
• Replicon: chromosome
• Accession: NZ_CP072853
• Organism: Leptospira interrogans strain UI29382

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J9305_RS13395
• Protein ID: WP_000281044.1
• Coordinates: 3250619..3251017 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8F7E3
• Locus tag: J9305_RS13390
• Protein ID: WP_001192482.1
• Coordinates: 3250389..3250619 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9305_RS13355 (J9305_13285)	3245969..3246121	+	153	WP_000252425.1	hypothetical protein	-
J9305_RS13360 (J9305_13290)	3246174..3246500	+	327	WP_232616567.1	tyrosine-type recombinase/integrase	-
J9305_RS13365 (J9305_13295)	3246595..3247617	+	1023	WP_000288518.1	hypothetical protein	-
J9305_RS13370 (J9305_13300)	3247841..3248369	+	529	Protein_2644	hypothetical protein	-
J9305_RS13375 (J9305_13305)	3248648..3248983	+	336	WP_000669387.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J9305_RS13380 (J9305_13310)	3248980..3249279	+	300	WP_000769809.1	helix-turn-helix transcriptional regulator	-
J9305_RS13385	3249400..3249780	+	381	WP_000639169.1	hypothetical protein	-
J9305_RS13390 (J9305_13320)	3250389..3250619	+	231	WP_001192482.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J9305_RS13395 (J9305_13325)	3250619..3251017	+	399	WP_000281044.1	type II toxin-antitoxin system VapC family toxin	Toxin
J9305_RS13400 (J9305_13330)	3251115..3251414	+	300	Protein_2650	hypothetical protein	-
J9305_RS13405	3251436..3251663	+	228	Protein_2651	hypothetical protein	-
J9305_RS13410 (J9305_13335)	3251805..3252881	+	1077	WP_001170568.1	hypothetical protein	-
J9305_RS13415 (J9305_13340)	3252882..3253340	+	459	WP_001239009.1	GNAT family N-acetyltransferase	-
J9305_RS13420 (J9305_13345)	3253556..3254416	-	861	WP_000682876.1	hypothetical protein	-
J9305_RS13425 (J9305_13350)	3254957..3255724	-	768	WP_000636955.1	DUF1554 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15137.79 Da
• Isoelectric Point: 9.6893

>T199016 WP_000281044.1 NZ_CP072853:3250619-3251017 [Leptospira interrogans]
MYLLDTNICIFLIKKKNAVLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Nucleotide

• Length: 399 bp

>T199016 NZ_CP097107:422408-423733 [Citrobacter freundii]
ATGAGCCGGAAACAGCAACGTCTGGTAATTTGGATGAACGGCATCAAAGTCGGGTACTGGGAAAAAAGCAAAGGGGTAGA
CAGCTTAGAATACCTTCCTGAATGGGTTGCTGACGAACAGGGAAGGCCGCTGTCATTGTCCCTTCCTTTTACTCCCGGGA
ACCAGGTCTGGCGTGGAAATGTGGTACGTGACTATTTTGATAATTTACTCCCGGACAGCGAAGGCATACGCAGACGTCTG
GCAATGCGTTACAAGGCCGATAGCCTGGAGCCTTTTGATCTGCTGACGGAGCTGGGAAAAGACTGCGTGGGCGCGATACA
GCTGCTTCATGACGGAGATGAACCCACTGATTTATATTCCGTGAAGTATCACCCGCTTACTGAATCAGAAATTGCGGCAA
CTTTGCGTAATACCACGGAGACATTGCTACCGGGCAGGCCAGAAGATAACGACGACTTACGTTTATCTATTGCCGGTGCT
CAGGAGAAAACAGCCCTACTGTGGTATGAAGATCGGTGGTGTATGCCGGAGGGTAATACACCAACGACGCATATATTCAA
GCTACCGCTCGGACTTGTGGGGAACATGAAAGCCGACATGAGCTCGTCGGTTGAAAATGAATGGCTCTGCTCGGTACTTC
TCGACCAGTACGGGCTCCCCGTGGCAAGAACGCAGATTGCTCACTTTGAAGATCAGAAAGCGCTGGTGGTAGAACGTTTT
GACCGAAAATGGTCAGGAGACGGACAATGGATCATTCGTTTACCCCAGGAGGATATGTGTCAGGCCCTGGGCGTTTCGCC
ATTACGAAAATATCAGGCGGACGGCGGGCCAGGTATATCGGAAATTATGGAGGTTCTGAGTAATTCAGACCGTGCTGAGC
GTGACAAAGCGCAGTTTTTTATGACGCAGATAATTTTCTGGATGATGGCCGCAACAGATGGCCACGCTAAAAATTTCAGT
ATTTCTATTGGGCCACAGGGACGTTATCACCTTACACCAAACTATGATGTGTTGTCGGCATGGCCGGTGATCGGTCATGG
GAACAATCAGATATCTTGGCAGAAGTGCAAACTGGCGATGGCCGTACGTGGCAGTAGCAACTATTACCAGATATATCGAA
TACAACGACGCCACTGGATCAGGCATGGTGAGATAACCGGCCTGAGTAAACAGCAGACAGAGGCGATGATAGAGGAAATC
ATCGCCAGAACCCCGGGTGTCATTGAACGTGTTAGCGGGCTTCTTCCGGACCAGTTCCCCCAGCAGCTTGCGGAAAGTAT
TTTTGATGGAATGAGGCAGCAATGCAGGCGCCTGGCTGAGAAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8771.05 Da
• Isoelectric Point: 8.6588

>AT199016 WP_001192482.1 NZ_CP072853:3250389-3250619 [Leptospira interrogans]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Nucleotide

• Length: 231 bp

>AT199016 NZ_CP097107:422110-422403 [Citrobacter freundii]
ATCAATAATGATTACCCGTTAAACACGCTCAACCAGTTGCGTCCCTTGCTCATTGGTTTTCGCAAGGCGAATGGCCTGAC
GCAAAAAGACCTGTCCGAAAGGTTAGGTGTCACTCAACAGACATACTCACGTCTGGAAGCCAACCCTGCCAGTGCGAGCA
TTGAACGGCTATTCAAGGTGTTTTCCGTCCTGGGTGTAAAAATCAGCTTCTCCTCGGCAACCACTTCTTCAGAGAGGAAG
CAGACGGAAGAAATATATAAATTAAATTCACCTGCACGACAGGAGGATTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0F6HAF7