Detailed information of TA system TA199014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 525059..525695
• Replicon: chromosome
• Accession: NZ_CP072851
• Organism: Bacillus altitudinis strain 6ww6

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K2MHT4
• Locus tag: J9319_RS02530
• Protein ID: WP_003214169.1
• Coordinates: 525345..525695 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W8QJ31
• Locus tag: J9319_RS02525
• Protein ID: WP_003214273.1
• Coordinates: 525059..525340 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9319_RS02505 (J9319_02505)	521213..521818	-	606	WP_007496491.1	rhomboid family intramembrane serine protease	-
J9319_RS02510 (J9319_02510)	521913..522278	+	366	WP_017358398.1	holo-ACP synthase	-
J9319_RS02515 (J9319_02515)	522439..523455	+	1017	WP_007496489.1	outer membrane lipoprotein-sorting protein	-
J9319_RS02520 (J9319_02520)	523584..524765	+	1182	WP_108611670.1	alanine racemase	-
J9319_RS02525 (J9319_02525)	525059..525340	+	282	WP_003214273.1	hypothetical protein	Antitoxin
J9319_RS02530 (J9319_02530)	525345..525695	+	351	WP_003214169.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
J9319_RS02535 (J9319_02535)	525810..526640	+	831	WP_007496484.1	RsbT co-antagonist protein RsbRA	-
J9319_RS02540 (J9319_02540)	526645..527013	+	369	WP_003214235.1	RsbT antagonist protein RsbS	-
J9319_RS02545 (J9319_02545)	527016..527417	+	402	WP_003214085.1	anti-sigma regulatory factor	-
J9319_RS02550 (J9319_02550)	527428..528435	+	1008	WP_008346904.1	PP2C family protein-serine/threonine phosphatase	-
J9319_RS02555 (J9319_02555)	528495..528824	+	330	WP_007496474.1	anti-sigma factor antagonist	-
J9319_RS02560 (J9319_02560)	528821..529309	+	489	WP_210552309.1	anti-sigma B factor RsbW	-
J9319_RS02565 (J9319_02565)	529275..530063	+	789	WP_012009000.1	RNA polymerase sigma factor SigB	-
J9319_RS02570 (J9319_02570)	530063..530662	+	600	WP_017358391.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.99 Da
• Isoelectric Point: 5.1663

>T199014 WP_003214169.1 NZ_CP072851:525345-525695 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Nucleotide

• Length: 351 bp

>T199014 NZ_CP097105:c45397-45044 [Pseudomonas sp. NY11382]
ATGAGCAAGCGCCCGCAGCATCAATTCGACCGCGGTGACATCGTCTCCCTGGACATGGGTAGCGCTTCAACGCAGGTGGC
CTGTAAAGCGCTCGTGCTTTCCCCAGCAGCGTACAACGCCCTGGGGCTCGCCCTAGCCGTTCCTATCACCGAGCACGACA
GCAGCCGCTACGCCGGCTTTGCCGTCGCGATCGTCCTTCCGGGGCGCACGCCGGCGAGCGGGGCAGCCTTGGTCAACCTG
GTGCGGCCGGTGGATCTTGCTGCAAGAGGTGCGAAGCTGCTCGGGAAGGCACCCCAGACAACCATAGATGAAGCCCTGCA
GCGCCTGCAGGCGGTTGTTGGGAAAGACCAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10864.25 Da
• Isoelectric Point: 5.2726

>AT199014 WP_003214273.1 NZ_CP072851:525059-525340 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT199014 NZ_CP097105:c45642-45394 [Pseudomonas sp. NY11382]
TTGAAAACGACTATCCGTGCGATTGGGAATTCTCAGGGCATCACGATACCCAAGCCGTTGCTGGCCGAACTCGGCGTAAG
CCTTGGCGACCCTGTAGACGTCTCCTTCAAAGATCAGACCCTGGTGGTGTCCAAGGTCGCTACCGAATACGACCTCAGCA
CGCTAGTCGCCCAGTGTGACCTGGAGGCCCCGGAGCCGCCGGACATCCACTGTTGGAGAACATCCGCGCCGGTAGGAAAC
GAAGCATGA

Structures

Toxin

• AlphaFold DB: K2MHT4

Antitoxin

• AlphaFold DB: A0A081L854