Detailed information of TA system TA198977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 599961..601786
• Replicon: chromosome
• Accession: NZ_CP072847
• Organism: Vibrio cholerae strain A1552

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0K9UVP8
• Locus tag: J9265_RS02830
• Protein ID: WP_000392003.1
• Coordinates: 600425..601786 (-)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J9265_RS02825
• Protein ID: WP_000804849.1
• Coordinates: 599961..600428 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9265_RS02810 (J9265_02810)	595241..595945	-	705	WP_001880605.1	nuclease-related domain-containing protein	-
J9265_RS02815 (J9265_02815)	596015..598951	-	2937	WP_001283872.1	DEAD/DEAH box helicase family protein	-
J9265_RS02820 (J9265_02820)	598955..599533	-	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
J9265_RS02825 (J9265_02825)	599961..600428	-	468	WP_000804849.1	helix-turn-helix transcriptional regulator	Antitoxin
J9265_RS02830 (J9265_02830)	600425..601786	-	1362	WP_000392003.1	type II toxin-antitoxin system HipA family toxin	Toxin
J9265_RS02835 (J9265_02835)	602390..603367	+	978	WP_001889101.1	IS481-like element ISVch1 family transposase	-
J9265_RS02840 (J9265_02840)	603393..604073	-	681	Protein_510	IS5 family transposase	-
J9265_RS02845 (J9265_02845)	604415..605935	-	1521	WP_000640055.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51246.17 Da
• Isoelectric Point: 4.9109

>T198977 WP_000392003.1 NZ_CP072847:c601786-600425 [Vibrio cholerae]
MEELTVQAFIRGEWIDIGIISFPKSSQHNFRVTELNYLSDYALEHYDKDDFHAVSLNHPVSFIFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSSHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWGLL

Nucleotide

• Length: 1362 bp

>T198977 NZ_CP097079:1896405-1896507 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17095.51 Da
• Isoelectric Point: 10.6157

>AT198977 WP_000804849.1 NZ_CP072847:c600428-599961 [Vibrio cholerae]
MKPSNKESVQDTVARLRENRNQQQSKVGKNATHSSKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT198977 NZ_CP097079:c1896514-1896369 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A0K9UVP8