Detailed information of TA system TA198974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 511526..512162
• Replicon: chromosome
• Accession: NZ_CP072845
• Organism: Bacillus subtilis strain XP

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: J9312_RS02630
• Protein ID: WP_003156187.1
• Coordinates: 511812..512162 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: J9312_RS02625
• Protein ID: WP_003225183.1
• Coordinates: 511526..511807 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9312_RS02605 (J9312_02595)	507885..508484	-	600	WP_041850724.1	rhomboid family intramembrane serine protease	-
J9312_RS02610 (J9312_02600)	508579..508944	+	366	WP_069963989.1	holo-ACP synthase	-
J9312_RS02615 (J9312_02605)	509110..510126	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
J9312_RS02620 (J9312_02610)	510241..511410	+	1170	WP_015252766.1	alanine racemase	-
J9312_RS02625 (J9312_02615)	511526..511807	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
J9312_RS02630 (J9312_02620)	511812..512162	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
J9312_RS02635 (J9312_02625)	512278..513102	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
J9312_RS02640 (J9312_02630)	513107..513472	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
J9312_RS02645 (J9312_02635)	513476..513877	+	402	WP_017697001.1	serine/threonine-protein kinase RsbT	-
J9312_RS02650 (J9312_02640)	513889..514896	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
J9312_RS02655 (J9312_02645)	514958..515287	+	330	WP_014662855.1	anti-sigma factor antagonist RsbV	-
J9312_RS02660 (J9312_02650)	515284..515766	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
J9312_RS02665 (J9312_02655)	515732..516520	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
J9312_RS02670 (J9312_02660)	516520..517119	+	600	WP_021481701.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T198974 WP_003156187.1 NZ_CP072845:511812-512162 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T198974 NZ_CP097079:326593-326961 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCTGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT198974 WP_003225183.1 NZ_CP072845:511526-511807 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT198974 NZ_CP097079:326375-326596 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32