Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 5088161..5088971 Replicon chromosome
Accession NZ_CP072826
Organism Klebsiella pneumoniae strain IRQBAS105c

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag J9263_RS24880 Protein ID WP_004178461.1
Coordinates 5088161..5088694 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag J9263_RS24885 Protein ID WP_002887278.1
Coordinates 5088705..5088971 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9263_RS24850 (5083455) 5083455..5083997 + 543 WP_004145330.1 type I DNA topoisomerase -
J9263_RS24855 (5084002) 5084002..5084574 + 573 WP_002919132.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
J9263_RS24860 (5084578) 5084578..5085396 + 819 WP_002919126.1 shikimate dehydrogenase -
J9263_RS24865 (5085393) 5085393..5085650 + 258 WP_002919125.1 DUF1488 domain-containing protein -
J9263_RS24870 (5085626) 5085626..5086180 - 555 WP_117260286.1 gamma carbonic anhydrase family protein -
J9263_RS24875 (5086992) 5086992..5088113 + 1122 WP_009309849.1 cupin domain-containing protein -
J9263_RS24880 (5088161) 5088161..5088694 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
J9263_RS24885 (5088705) 5088705..5088971 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
J9263_RS24890 (5089074) 5089074..5090507 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
J9263_RS24895 (5090497) 5090497..5091180 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
J9263_RS24900 (5091353) 5091353..5092738 + 1386 WP_087793843.1 efflux transporter outer membrane subunit -
J9263_RS24905 (5092756) 5092756..5093100 + 345 WP_048266266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T198960 WP_004178461.1 NZ_CP072826:c5088694-5088161 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T198960 NZ_CP097076:352564-352911 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATTCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGAGCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT198960 WP_002887278.1 NZ_CP072826:c5088971-5088705 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT198960 NZ_CP097076:352911-353210 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References