Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4831961..4832586 Replicon chromosome
Accession NZ_CP072826
Organism Klebsiella pneumoniae strain IRQBAS105c

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J9263_RS23555 Protein ID WP_002882817.1
Coordinates 4832203..4832586 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J9263_RS23550 Protein ID WP_004150355.1
Coordinates 4831961..4832203 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9263_RS23525 (4827952) 4827952..4828551 + 600 WP_004151865.1 glucose-1-phosphatase -
J9263_RS23530 (4828545) 4828545..4829405 + 861 WP_004146232.1 virulence factor BrkB family protein -
J9263_RS23535 (4829402) 4829402..4829839 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
J9263_RS23540 (4829884) 4829884..4830825 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J9263_RS23545 (4830839) 4830839..4831756 - 918 WP_117260249.1 alpha/beta hydrolase -
J9263_RS23550 (4831961) 4831961..4832203 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
J9263_RS23555 (4832203) 4832203..4832586 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J9263_RS23560 (4832760) 4832760..4833689 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J9263_RS23565 (4833686) 4833686..4834321 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J9263_RS23570 (4834318) 4834318..4835220 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T198958 WP_002882817.1 NZ_CP072826:4832203-4832586 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T198958 NZ_CP097071:48416-48724 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT198958 WP_004150355.1 NZ_CP072826:4831961-4832203 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT198958 NZ_CP097071:48142-48414 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References