Detailed information of TA system TA198942

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 5472325..5473235
• Replicon: chromosome
• Accession: NZ_CP072825
• Organism: Klebsiella pneumoniae strain IRQBAS103c

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: J9262_RS26940
• Protein ID: WP_004181896.1
• Coordinates: 5472765..5473235 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: J9262_RS26935
• Protein ID: WP_004181895.1
• Coordinates: 5472325..5472768 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9262_RS26895 (5467518)	5467518..5467877	+	360	Protein_5299	IS1 family transposase	-
J9262_RS26900 (5468667)	5468667..5468993	-	327	WP_004201125.1	C2H2-type zinc finger protein	-
J9262_RS26905 (5469175)	5469175..5469456	+	282	WP_001568067.1	hypothetical protein	-
J9262_RS26910 (5469510)	5469510..5470121	+	612	WP_000176305.1	DUF2913 family protein	-
J9262_RS26915 (5470118)	5470118..5470228	-	111	Protein_5303	hypothetical protein	-
J9262_RS26920 (5470270)	5470270..5470377	-	108	Protein_5304	IS6 family transposase	-
J9262_RS26925 (5470531)	5470531..5470953	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
J9262_RS26930 (5470953)	5470953..5472224	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
J9262_RS26935 (5472325)	5472325..5472768	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
J9262_RS26940 (5472765)	5472765..5473235	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
J9262_RS26945 (5473345)	5473345..5473605	-	261	WP_206552253.1	hypothetical protein	-
J9262_RS26950 (5474304)	5474304..5475673	+	1370	WP_085949440.1	IS3-like element ISKpn8 family transposase	-
J9262_RS26955 (5475850)	5475850..5476560	+	711	Protein_5311	ISNCY family transposase	-
J9262_RS26960 (5476815)	5476815..5477888	-	1074	Protein_5312	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / aac(3)-IId / tet(B)	tufA	5291619..5486510	194891

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T198942 WP_004181896.1 NZ_CP072825:5472765-5473235 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T198942 NZ_CP097066:c2571508-2571365 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT198942 WP_004181895.1 NZ_CP072825:5472325-5472768 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT198942 NZ_CP097066:2571246-2571391 [Enterococcus faecalis]
TGTGCTATAATGAAAATGAAAAGAGAGATATGCTTCAACATACCTCTCTAGTGTAGAGCCGTTTGAGACGGTGACCGATT
ATATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTATTGTCATTTTTAAGCAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9