198941

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 5376942..5377752 Replicon chromosome
Accession NZ_CP072825
Organism Klebsiella pneumoniae strain IRQBAS103c

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag J9262_RS26385 Protein ID WP_004178461.1
Coordinates 5376942..5377475 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag J9262_RS26390 Protein ID WP_002887278.1
Coordinates 5377486..5377752 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9262_RS26365 (5372915) 5372915..5374321 - 1407 Protein_5192 Tn3-like element IS3000 family transposase -
J9262_RS26370 (5374596) 5374596..5374931 + 336 Protein_5193 IS6-like element IS26 family transposase -
J9262_RS26375 (5374985) 5374985..5375230 - 246 Protein_5194 class II aldolase/adducin family protein -
J9262_RS26380 (5375773) 5375773..5376894 + 1122 WP_009309849.1 cupin domain-containing protein -
J9262_RS26385 (5376942) 5376942..5377475 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
J9262_RS26390 (5377486) 5377486..5377752 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
J9262_RS26395 (5377855) 5377855..5379288 - 1434 WP_023280821.1 Cu(+)/Ag(+) sensor histidine kinase -
J9262_RS26400 (5379278) 5379278..5379961 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
J9262_RS26405 (5380134) 5380134..5381519 + 1386 WP_087793843.1 efflux transporter outer membrane subunit -
J9262_RS26410 (5381537) 5381537..5381881 + 345 WP_048266266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib / aac(3)-IId / tet(B) tufA 5291619..5486510 194891


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T198941 WP_004178461.1 NZ_CP072825:c5377475-5376942 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T198941 NZ_CP097066:c2571133-2570990 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT198941 WP_002887278.1 NZ_CP072825:c5377752-5377486 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT198941 NZ_CP097066:2571234-2571432 [Enterococcus faecalis]
TTTTTAGGGAAATGTGCTATAATGAAAATGAAAAGAGAGATATGCTTCAACATACCTCTCTAGTGTAGAGCCGTTTGAGA
CGGTGACCGATTATATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTATTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLZ1

References