Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 5010483..5011009 Replicon chromosome
Accession NZ_CP072825
Organism Klebsiella pneumoniae strain IRQBAS103c

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag J9262_RS24390 Protein ID WP_000323025.1
Coordinates 5010722..5011009 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag J9262_RS24385 Protein ID WP_000534858.1
Coordinates 5010483..5010722 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J9262_RS24365 (5005755) 5005755..5006390 + 636 Protein_4790 transposase -
J9262_RS24370 (5006811) 5006811..5008049 + 1239 WP_003030308.1 IS110 family transposase -
J9262_RS24375 (5008525) 5008525..5009097 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
J9262_RS24380 (5009297) 5009297..5010220 + 924 WP_020277922.1 cation diffusion facilitator family transporter -
J9262_RS27580 (5010354) 5010354..5010458 - 105 Protein_4794 hypothetical protein -
J9262_RS24385 (5010483) 5010483..5010722 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
J9262_RS24390 (5010722) 5010722..5011009 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
J9262_RS24395 (5011081) 5011081..5011239 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
J9262_RS24400 (5011843) 5011843..5012817 + 975 WP_210502162.1 Hok/Gef family protein -
J9262_RS24405 (5013015) 5013015..5013392 - 378 WP_004181901.1 hypothetical protein -
J9262_RS24410 (5014081) 5014081..5015148 + 1068 WP_004181903.1 hypothetical protein -
J9262_RS24415 (5015304) 5015304..5015651 + 348 WP_004181904.1 hypothetical protein -
J9262_RS24420 (5015730) 5015730..5015963 + 234 WP_004181905.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 5009055..5101174 92119


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T198939 WP_000323025.1 NZ_CP072825:5010722-5011009 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T198939 NZ_CP097066:c2496628-2496527 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT198939 WP_000534858.1 NZ_CP072825:5010483-5010722 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT198939 NZ_CP097066:2496402-2496582 [Enterococcus faecalis]
TGAAAAGAGAGGTATGCGGGTACATACCTCTCCTTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAG
TTTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATAC
CGAAAATCAGTAGTGCAACAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References