Detailed information of TA system TA198935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 4181189..4181779
• Replicon: chromosome
• Accession: NZ_CP072825
• Organism: Klebsiella pneumoniae strain IRQBAS103c

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: J9262_RS20330
• Protein ID: WP_023341911.1
• Coordinates: 4181189..4181521 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A3S7DDD0
• Locus tag: J9262_RS20335
• Protein ID: WP_000288812.1
• Coordinates: 4181522..4181779 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J9262_RS20305 (4177236)	4177236..4177481	+	246	WP_023284006.1	signal transduction protein PmrD	-
J9262_RS20310 (4177611)	4177611..4179065	+	1455	WP_004148975.1	AMP nucleosidase	-
J9262_RS20320 (4179431)	4179431..4180867	-	1437	WP_004151469.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
J9262_RS20330 (4181189)	4181189..4181521	-	333	WP_023341911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J9262_RS20335 (4181522)	4181522..4181779	-	258	WP_000288812.1	antitoxin	Antitoxin
J9262_RS20340 (4182114)	4182114..4182320	-	207	WP_117259919.1	helix-turn-helix transcriptional regulator	-
J9262_RS20345 (4182317)	4182317..4182777	-	461	Protein_3996	hypothetical protein	-
J9262_RS20350 (4183060)	4183060..4183608	-	549	WP_117259920.1	hypothetical protein	-
J9262_RS20355 (4183687)	4183687..4185435	-	1749	WP_096106564.1	hypothetical protein	-
J9262_RS27535 (4185786)	4185786..4185863	-	78	Protein_3999	type II toxin-antitoxin system PemK/MazF family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11927.87 Da
• Isoelectric Point: 10.4722

>T198935 WP_023341911.1 NZ_CP072825:c4181521-4181189 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARKGKRLERIPDVVVNEVLARLDAMLS

Nucleotide

• Length: 333 bp

>T198935 NZ_CP097064:24038-24901 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAGTTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAGCTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCAAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9442.92 Da
• Isoelectric Point: 4.3566

>AT198935 WP_000288812.1 NZ_CP072825:c4181779-4181522 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT198935 NZ_CP097064:23764-24036 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGAATATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A3S7DDD0