Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 64609..65144 Replicon plasmid pPK5074-91kb
Accession NZ_CP072806
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name relE Uniprot ID A0A0K4CX11
Locus tag J8617_RS25255 Protein ID WP_000222771.1
Coordinates 64609..64896 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T1PXV2
Locus tag J8617_RS25260 Protein ID WP_001139206.1
Coordinates 64893..65144 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS25245 (J8617_25230) 59784..63299 + 3516 WP_165463807.1 autotransporter outer membrane beta-barrel domain-containing protein -
J8617_RS25250 (J8617_25235) 63531..64227 + 697 WP_232379233.1 IS1-like element IS1B family transposase -
J8617_RS25255 (J8617_25240) 64609..64896 - 288 WP_000222771.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J8617_RS25260 (J8617_25245) 64893..65144 - 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J8617_RS25265 (J8617_25250) 65757..67573 + 1817 Protein_72 helicase -
J8617_RS25270 (J8617_25255) 67607..68047 + 441 WP_038997035.1 hypothetical protein -
J8617_RS25275 (J8617_25260) 68044..68292 + 249 WP_000747846.1 hypothetical protein -
J8617_RS25280 (J8617_25265) 68560..69186 - 627 Protein_75 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / sul2 / qnrS2 / floR / tet(A) - 1..91224 91224
- inside IScluster/Tn qnrS2 / floR / tet(A) - 63531..89996 26465


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11116.17 Da        Isoelectric Point: 10.5833

>T198919 WP_000222771.1 NZ_CP072806:c64896-64609 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Download         Length: 288 bp

>T198919 NZ_CP097059:c320472-320377 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9171.39 Da        Isoelectric Point: 4.0927

>AT198919 WP_001139206.1 NZ_CP072806:c65144-64893 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Download         Length: 252 bp

>AT198919 NZ_CP097059:320278-320342 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATTGTGGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CX11

Antitoxin

Source ID Structure
AlphaFold DB T1PXV2

References