198918

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 38114..38640 Replicon plasmid pPK5074-91kb
Accession NZ_CP072806
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag J8617_RS25135 Protein ID WP_000323025.1
Coordinates 38353..38640 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag J8617_RS25130 Protein ID WP_000534858.1
Coordinates 38114..38353 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS25100 (J8617_25085) 33501..34084 + 584 Protein_36 IS1 family transposase -
J8617_RS25105 (J8617_25090) 34148..34852 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
J8617_RS25110 (J8617_25095) 35195..35719 - 525 WP_232379235.1 Ig-like domain-containing protein -
J8617_RS25115 (J8617_25100) 35725..36429 - 705 WP_001067852.1 IS6-like element IS26 family transposase -
J8617_RS25120 (J8617_25105) 36506..37579 - 1074 WP_000557466.1 hypothetical protein -
J8617_RS25125 (J8617_25110) 37623..37772 - 150 WP_000550473.1 hypothetical protein -
J8617_RS26430 37979..38089 - 111 Protein_42 protein YdfV -
J8617_RS25130 (J8617_25115) 38114..38353 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
J8617_RS25135 (J8617_25120) 38353..38640 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
J8617_RS25140 (J8617_25125) 38923..39147 + 225 Protein_45 hypothetical protein -
J8617_RS25145 (J8617_25130) 39584..39793 + 210 WP_072662162.1 hypothetical protein -
J8617_RS25150 (J8617_25135) 39799..40288 - 490 Protein_47 IS3 family transposase -
J8617_RS25155 (J8617_25140) 40369..41877 - 1509 WP_141223309.1 group II intron reverse transcriptase/maturase -
J8617_RS25160 (J8617_25145) 42550..42975 - 426 Protein_49 IS3 family transposase -
J8617_RS26435 42963..43043 + 81 Protein_50 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / sul2 / qnrS2 / floR / tet(A) - 1..91224 91224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T198918 WP_000323025.1 NZ_CP072806:38353-38640 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T198918 NZ_CP097058:c57275-56412 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT198918 WP_000534858.1 NZ_CP072806:38114-38353 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT198918 NZ_CP097058:c57549-57277 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References