Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 38114..38640 Replicon plasmid pPK5074-91kb
Accession NZ_CP072806
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag J8617_RS25135 Protein ID WP_000323025.1
Coordinates 38353..38640 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag J8617_RS25130 Protein ID WP_000534858.1
Coordinates 38114..38353 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS25100 (J8617_25085) 33501..34084 + 584 Protein_36 IS1 family transposase -
J8617_RS25105 (J8617_25090) 34148..34852 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
J8617_RS25110 (J8617_25095) 35195..35719 - 525 WP_232379235.1 Ig-like domain-containing protein -
J8617_RS25115 (J8617_25100) 35725..36429 - 705 WP_001067852.1 IS6-like element IS26 family transposase -
J8617_RS25120 (J8617_25105) 36506..37579 - 1074 WP_000557466.1 hypothetical protein -
J8617_RS25125 (J8617_25110) 37623..37772 - 150 WP_000550473.1 hypothetical protein -
J8617_RS26430 37979..38089 - 111 Protein_42 protein YdfV -
J8617_RS25130 (J8617_25115) 38114..38353 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
J8617_RS25135 (J8617_25120) 38353..38640 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
J8617_RS25140 (J8617_25125) 38923..39147 + 225 Protein_45 hypothetical protein -
J8617_RS25145 (J8617_25130) 39584..39793 + 210 WP_072662162.1 hypothetical protein -
J8617_RS25150 (J8617_25135) 39799..40288 - 490 Protein_47 IS3 family transposase -
J8617_RS25155 (J8617_25140) 40369..41877 - 1509 WP_141223309.1 group II intron reverse transcriptase/maturase -
J8617_RS25160 (J8617_25145) 42550..42975 - 426 Protein_49 IS3 family transposase -
J8617_RS26435 42963..43043 + 81 Protein_50 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / sul2 / qnrS2 / floR / tet(A) - 1..91224 91224


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T198918 WP_000323025.1 NZ_CP072806:38353-38640 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T198918 NZ_CP097058:c57275-56412 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT198918 WP_000534858.1 NZ_CP072806:38114-38353 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT198918 NZ_CP097058:c57549-57277 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References