Detailed information of TA system TA198917

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 12945..13546
• Replicon: plasmid pPK5074-91kb
• Accession: NZ_CP072806
• Organism: Escherichia coli strain PK5074

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: J8617_RS25010
• Protein ID: WP_001216039.1
• Coordinates: 12945..13325 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: J8617_RS25015
• Protein ID: WP_001190711.1
• Coordinates: 13325..13546 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J8617_RS24975 (J8617_24960)	8007..9038	-	1032	WP_000067534.1	site-specific integrase	-
J8617_RS24980 (J8617_24965)	9046..9267	-	222	WP_000542385.1	hypothetical protein	-
J8617_RS24985 (J8617_24970)	9872..10081	+	210	WP_000874156.1	hypothetical protein	-
J8617_RS24990 (J8617_24975)	10192..11043	+	852	WP_000611661.1	phage repressor protein C1	-
J8617_RS24995 (J8617_24980)	11076..11450	-	375	WP_000028983.1	phage antirepressor KilAC domain-containing protein	-
J8617_RS25000 (J8617_24985)	11690..12733	-	1044	WP_000648838.1	DUF968 domain-containing protein	-
J8617_RS25005 (J8617_24990)	12761..12940	-	180	WP_000113018.1	hypothetical protein	-
J8617_RS25010 (J8617_24995)	12945..13325	-	381	WP_001216039.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
J8617_RS25015 (J8617_25000)	13325..13546	-	222	WP_001190711.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
J8617_RS25020 (J8617_25005)	13811..14508	+	698	WP_223400810.1	IS1-like element IS1X2 family transposase	-
J8617_RS25025 (J8617_25010)	14525..14851	-	327	Protein_20	hypothetical protein	-
J8617_RS25030 (J8617_25015)	14924..16186	-	1263	WP_210388690.1	hypothetical protein	-
J8617_RS25035 (J8617_25020)	16488..17189	-	702	WP_000684873.1	hypothetical protein	-
J8617_RS25040 (J8617_25025)	17186..17350	-	165	Protein_23	serine/threonine protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / qnrS2 / floR / tet(A)	-	1..91224	91224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13558.27 Da
• Isoelectric Point: 5.1514

>T198917 WP_001216039.1 NZ_CP072806:c13325-12945 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRAALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T198917 NZ_CP097058:c42346-42005 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8134.08 Da
• Isoelectric Point: 4.5843

>AT198917 WP_001190711.1 NZ_CP072806:c13546-13325 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVDR

Nucleotide

• Length: 222 bp

>AT198917 NZ_CP097058:c42576-42346 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA