Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 30098..30645 Replicon plasmid pPK5074-69kb
Accession NZ_CP072804
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag J8617_RS24730 Protein ID WP_001384452.1
Coordinates 30098..30376 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A1M1SX19
Locus tag J8617_RS24735 Protein ID WP_021546940.1
Coordinates 30376..30645 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS24695 (J8617_24680) 25239..25718 + 480 WP_130560492.1 type 3 dihydrofolate reductase -
J8617_RS24700 (J8617_24685) 25739..25918 + 180 WP_000998543.1 type II toxin-antitoxin system CcdA family antitoxin -
J8617_RS24705 (J8617_24690) 26017..26436 - 420 WP_044866469.1 hypothetical protein -
J8617_RS24710 (J8617_24695) 26537..27234 + 698 WP_223429336.1 IS1 family transposase -
J8617_RS24715 (J8617_24700) 27435..28295 + 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
J8617_RS24720 (J8617_24705) 28605..29087 - 483 WP_210388685.1 hypothetical protein -
J8617_RS24725 (J8617_24710) 29204..30052 - 849 WP_001058012.1 3'-5' exonuclease -
J8617_RS24730 (J8617_24715) 30098..30376 - 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
J8617_RS24735 (J8617_24720) 30376..30645 - 270 WP_021546940.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
J8617_RS24740 (J8617_24725) 31392..32003 + 612 Protein_43 IS3 family transposase -
J8617_RS24745 (J8617_24730) 32035..32214 - 180 WP_012600012.1 hypothetical protein -
J8617_RS24750 (J8617_24735) 32438..32755 + 318 WP_032156934.1 hypothetical protein -
J8617_RS24755 (J8617_24740) 32824..33750 + 927 WP_000617209.1 WYL domain-containing protein -
J8617_RS24760 (J8617_24745) 33760..34359 + 600 WP_001021938.1 DUF1819 family protein -
J8617_RS24765 (J8617_24750) 34356..34940 + 585 WP_000645940.1 DUF1788 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid lnu(F) / aadA2 / qnrS1 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / blaTEM-1B - 1..69302 69302
- inside IScluster/Tn lnu(F) / aadA2 / qnrS1 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / blaTEM-1B - 3929..32003 28074


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T198916 WP_001384452.1 NZ_CP072804:c30376-30098 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T198916 NZ_CP097057:35566-35841 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAATT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCATGCACTAACAGGAAATTATA
TAGGTACACGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCCCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10321.69 Da        Isoelectric Point: 8.8725

>AT198916 WP_021546940.1 NZ_CP072804:c30645-30376 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMRRKVADK

Download         Length: 270 bp

>AT198916 NZ_CP097057:35307-35573 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A1M1SX19

References