Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 90174..91797 Replicon plasmid pPK5074-MCR1
Accession NZ_CP072803
Organism Escherichia coli strain PK5074

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag J8617_RS23555 Protein ID WP_000088046.1
Coordinates 90472..91797 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag J8617_RS23550 Protein ID WP_024136327.1
Coordinates 90174..90467 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J8617_RS26640 85244..85375 + 132 WP_000374059.1 hypothetical protein -
J8617_RS23520 (J8617_23510) 85716..85997 - 282 WP_000077926.1 hypothetical protein -
J8617_RS23525 (J8617_23515) 86047..86238 - 192 WP_000398480.1 hypothetical protein -
J8617_RS23530 (J8617_23520) 86462..87634 + 1173 WP_000952349.1 IS21-like element IS21 family transposase -
J8617_RS23535 (J8617_23525) 87634..88431 + 798 WP_000544828.1 IS21-like element IS21 family helper ATPase IstB -
J8617_RS23540 (J8617_23530) 88470..88805 - 336 WP_065311125.1 hypothetical protein -
J8617_RS23545 (J8617_23535) 89178..89570 + 393 WP_000341066.1 hypothetical protein -
J8617_RS23550 (J8617_23540) 90174..90467 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
J8617_RS23555 (J8617_23545) 90472..91797 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
J8617_RS23560 (J8617_23550) 91858..92064 + 207 WP_000134171.1 hypothetical protein -
J8617_RS23565 (J8617_23555) 92166..92576 + 411 WP_000985911.1 hypothetical protein -
J8617_RS23570 (J8617_23560) 92589..93404 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
J8617_RS23575 (J8617_23565) 93658..94083 + 426 WP_001043843.1 hypothetical protein -
J8617_RS23580 (J8617_23570) 94632..94940 + 309 WP_001224687.1 hypothetical protein -
J8617_RS23585 (J8617_23575) 94989..95813 + 825 WP_174411902.1 HNH endonuclease signature motif containing protein -
J8617_RS23590 (J8617_23580) 95875..96078 + 204 WP_001194555.1 hypothetical protein -
J8617_RS23595 (J8617_23585) 96420..96776 - 357 WP_065311124.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-1.1 / aph(3')-Ia / ant(3'')-Ia / lnu(F) / sul3 / tet(A) / aph(6)-Id / floR / ARR-3 / dfrA14 - 1..267744 267744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T198914 WP_000088046.1 NZ_CP072803:90472-91797 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T198914 NZ_CP097056:2735976-2736119 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAACCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT198914 WP_024136327.1 NZ_CP072803:90174-90467 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT198914 NZ_CP097056:c2735865-2735719 [Enterococcus faecalis]
TTGTGCTATAATAGCAATGAAAAGAGAGATATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCTTT
TTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References